781665-66-5Relevant academic research and scientific papers
Synthesis of glyoxylyl peptides using a phosphine labile α,α′-diaminoacetic acid derivative
Far, Samia,Melnyk, Oleg
, p. 7163 - 7165 (2004)
We describe in this letter the preparation of a novel protected α,α′-diaminoacetic acid derivative that acts as a masked glyoxylic acid equivalent. The reagent could easily be introduced on a peptide chain using standard Fmoc/tert-butyl solid-phase methods and resisted to the TFA treatment allowing the deprotection and cleavage of the peptide. Unmasking of the glyoxylyl group was performed in solution in the presence of a phosphine.
A novel phosphoramidite for the synthesis of α-oxo aldehyde-modified oligodeoxynucleotides
Far, Samia,Gouyette, Catherine,Melnyk, Oleg
, p. 6138 - 6142 (2005)
The synthesis and use of a novel phosphoramidite derivatized by a bis[2-(tert-butyldisulfanyl)ethoxycarbonylamino]acetyl moiety for the synthesis of oligodeoxynucleotides modified at the 5′-end by an α-oxo aldehyde functionality is presented. Incorporation of the phosphoramidite reagent was performed after the automated solid-phase oligonucleotide synthesis. Simultaneous cleavage/deprotection of the oligodeoxynucleotides and unmasking of the α-oxo aldehyde group could be achieved using NaOH in aqueous methanol in the presence of dithiothreitol.
