79965-05-2Relevant academic research and scientific papers
Inhibitors of an AdoMet-dependent 3-amino-3-carboxypropyl transferase and their use as ligands for protein affinity chromatography
Reeve, Anne M.,Townsend, Craig A.
, p. 15959 - 15974 (1998)
The screening of potential affinity ligands and the development of a two-stage affinity purification of an S-adenosylmethionine-dependent 3- amino-3-carboxypropyl transferase involved in the biosynthesis of the β- lactam antibiotic nocardicin A is described from a cell-free extract of Nocardia uniformis subsp. tsuyamanesis. This protein is particularly sensitive to ion exchange and salt effects necessitating a pair of neutral ligands to be used in the final purification.
