Tetrahedron p. 15959 - 15974 (1998)
Update date:2022-08-04
Topics:
Reeve, Anne M.
Townsend, Craig A.
The screening of potential affinity ligands and the development of a two-stage affinity purification of an S-adenosylmethionine-dependent 3- amino-3-carboxypropyl transferase involved in the biosynthesis of the β- lactam antibiotic nocardicin A is described from a cell-free extract of Nocardia uniformis subsp. tsuyamanesis. This protein is particularly sensitive to ion exchange and salt effects necessitating a pair of neutral ligands to be used in the final purification.
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