80226-49-9

Upstream product

• 64-18-6 formic acid 
• 1004-75-7 2,5,6-triamino-4-hydroxypyrimidine 
• 2387-48-6 2,4-diamino6-hydroxy-5-nitroso pyrimidine 

Relevant academic research and scientific papers

Nitrogen-Doped Carbon Supported Co/Ni Bimetallic Catalyst for Selectively Reductive N-Formylation of Nitroso in Guanine Synthesis

A nitrogen-doped carbon supported Co/Ni bimetallic catalyst 2wt%Co/Ni@NC-700-10 was prepared and found to be efficient in the reductive N-formylation of 2,4-diamino-5-nitroso-6-hydroxypyrimidine (DANHP) to 2,4-diamino-5-formyl-6-hydroxypyrimidine (DAFHP) for the synthesis of guanine. Under optimal conditions, the conversion of DANHP reached up to 95.6% with a DAFHP selectivity of 97.6%. The characterization results revealed that the cobalt/nickel nanoparticles of the catalyst uniformly dispersed and encapsulated in the nitrogen-dopped carbon, and the catalyst has the characters of large surface area, as well as uniform dispersion and small diameters of metal nanoparticles in the form of Co/Ni alloy, which endowed its good catalytic performance in the reaction. Graphical abstract: [Figure not available: see fulltext.]

Simultaneous determination of five oxidative DNA lesions in human urine

A method, involving a HPLC prepurification followed by a GC/MS analysis, has been set up for the measurement of nucleic acid oxidation products in human urine. For this purpose, isotopically labeled internal standards have been prepared and used for isotope dilution mass spectrometric detection. Using this approach, four oxidized DNA bases, i.e., 5-hydroxyuracil, 5- (hydroxymethyl)uracil, 8-oxo-7,8-dihydroadenine, and 8-oxo-7,8- dihydroguanine, together with 8-oxo-7,8-dihydro-2'-deoxyguanosine have been simultaneously quantified in human urine samples. The levels of the oxidized nucleic acid constituents, as expressed in picomoles per milliliter, were determined to be, in decreasing order: 8-oxo-7,8-dihydroguanine (583 ± 376) > 5-(hydroxymethyl)uracil (121 ± 56) > 5-hydroxyuracil (58 ± 23) > 8-oxo- 7,8-dihydro-2'-deoxyguanosine (30 ± 15) > 8-oxo-7,8-dihydroadenine (7 ± 4). Attempts to determine the amount of 5,6-dihydroxy-5,6-dihydrothymine, 5- hydroxycytosine, and 2,6-diamino-4-hydroxy-5-formamidopyrimidine using the above HPLC-GC/MS method were unsuccessful.

Process for the preparation of TAHP

The invention relates to an improved process for the catalytic hydrogenation of DAHNP and a new process for preparing 2,4-diamino-5-formylamino-6-hydroxypyrimidine.