80614-95-5

Upstream product

• 81196-20-5 2-chlorophenyl 5'-O-(4,4'-dimethoxytrityl)thymidin-3'-yl phosphate 
• 50-89-5 thymidine 
• 91592-77-7 5’-O-(4,4’-dimethoxytrityl)thymidine 3’-(benzotrizol-1-yl 2-chlorophenyl phosphate) 
• 83866-30-2 3'-O-formyl-2'-deoxythymidine 
• 72351-28-1 2-chlorophenyl phosphordi(1H-1-[1,2,4]triazolide) 
• 40615-39-2 5'-O-(4-4'-dimethoxytrityl)thymidine 
• 80817-46-5 bis(benzotriazol-1-yl)(2-chlorophenyl)phosphate 
• 80614-94-4 C₄₈H₄₈ClN₄O₁₅P 

Downstream Products

• 80614-96-6 C₈₀H₈₀Cl₃N₈O₂₉P₃ 
• 111462-77-2 5'-O-(4,4'-dimethoxytrityl)thymidylyl-(3'-5')-(o-chlorophenyl)-thymidine 3'-(methyl)N,N-diisopropylphosphoramidite 

Relevant academic research and scientific papers

Efficient and flexible access to fully protected trinucleotides suitable for DNA synthesis by automated phosphoramidite chemistry

An efficient synthetic approach to protected trinucleotide phosphoramidites suitable for codon-by-codon synthesis of structural gene combinatorial libraries is described; the procedure rests on the use of a pair of orthogonal protecting groups for the two

SYNTHESIS OF DNA FRAGMENTS BY THE HYDROXYBENZOTRIAZOLE PHOSPHOTRIESTER APPROACH

The application of the phosphorylating agent 2-chlorophenyl-o,o-bis--phosphate (HOBT-approach) for the introduction of 3'-5'-phosphotriester linkages between a properly 5'-protected (4,4'-dimethoxytrityl) deoxy-nucleoside and a deoxy-nucleoside having free OH groups is described.In this way, all sixteen partially-protected DNA dimers having a free 3'-OH group have been synthesized.The dimers thus obtained have been applied, using the HOBT-approach, to the synthesis of the DNA fragments d-CGCGCG, d-CGTACG and d-ATATTAATAAC.

3'-O-FORMYL-(N-ACYL)-2'-DEOXYRIBONUCLEOSIDES AS BUILDING UNITS IN THE SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES

5'-O-Dimethoxytrityl-(N-acyl)-2'-deoxyribonucleosides afford 3'-O-formyl-(N-acyl)-2'-deoxyribonucleosides Ia-Id by the action of formic acetic anhydride followed by the action of 80percent aqueous acetic acid.The formyl group is removed from Ia-Id by treatment with 1 mol l-1 triethylamine. 3'-O-Formyl-2'-deoxythymidine (Ia) gives 3'-O-dimethoxytrityl-2'-deoxythymidine (V) by subsequent treatment with acetic anhydride, triethylamine, dimethoxytrityl chloride and methanolic ammonia.The use of compounds I for the synthesis of d-GGAGG (XIX) and d-T16 (XXXII) is described.Systems for thin-layer chromatography of 5'-O-dimethyltrityl-oligodeoxyribonucleotides on silica gel are described.