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N2-Isobutyryl-2''-O-(tert-butyldimethylsilyl)-5''-O-(4,4''-dimethoxytrityl)-gu is a modified nucleotide that is used in the preparation of 4''-C-methoxy-2''-deoxy-2''-fluoro modified ribonucleotides. It is characterized by the presence of an isobutyryl group at the N2 position, a tert-butyldimethylsilyl group at the 2'' position, and a 4,4''-dimethoxytrityl group at the 5'' position. These modifications are designed to improve the metabolic stability and efficiency of RNA interference (RNAi)-mediated gene silencing.

81279-39-2

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81279-39-2 Usage

Uses

Used in Pharmaceutical Industry:
N2-Isobutyryl-2''-O-(tert-butyldimethylsilyl)-5''-O-(4,4''-dimethoxytrityl)-gu is used as a key intermediate in the synthesis of modified ribonucleotides for the development of RNAi-based therapeutics. The modifications to the nucleotide enhance the metabolic stability and efficiency of RNAi-mediated gene silencing, which is crucial for the effectiveness of RNAi-based drugs.
Used in Research Applications:
N2-Isobutyryl-2''-O-(tert-butyldimethylsilyl)-5''-O-(4,4''-dimethoxytrityl)-gu is also used as a research tool in the study of RNAi mechanisms and the development of new RNAi-based technologies. The modified nucleotide can be incorporated into RNA molecules to investigate the effects of these modifications on RNA structure, stability, and function.
Used in Diagnostic Applications:
In addition to its use in therapeutics and research, N2-Isobutyryl-2''-O-(tert-butyldimethylsilyl)-5''-O-(4,4''-dimethoxytrityl)-gu may also have potential applications in the development of diagnostic tools. The modified nucleotide could be used to create probes for detecting specific RNA sequences or to develop assays for monitoring RNAi activity in biological samples.

Check Digit Verification of cas no

The CAS Registry Mumber 81279-39-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,1,2,7 and 9 respectively; the second part has 2 digits, 3 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 81279-39:
(7*8)+(6*1)+(5*2)+(4*7)+(3*9)+(2*3)+(1*9)=142
142 % 10 = 2
So 81279-39-2 is a valid CAS Registry Number.

81279-39-2SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 18, 2017

Revision Date: Aug 18, 2017

1.Identification

1.1 GHS Product identifier

Product name N-[9-[(2R,3R,4R,5R)-5-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-3-[tert-butyl(dimethyl)silyl]oxy-4-hydroxyoxolan-2-yl]-6-oxo-3H-purin-2-yl]-2-methylpropanamide

1.2 Other means of identification

Product number -
Other names 5'-dimethoxytrityl 2'-tert-butyldimethyl silyl N-isobutyrylguanosine

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:81279-39-2 SDS

81279-39-2Downstream Products

81279-39-2Relevant academic research and scientific papers

Practical silyl protection of ribonucleosides

Blaisdell, Thomas P.,Lee, Sunggi,Kasaplar, Pinar,Sun, Xixi,Tan, Kian L.

supporting information, p. 4710 - 4713 (2013/10/08)

Herein we report the site-selective silylation of the ribonucelosides. The method enables a simple and efficient procedure for accessing suitably protected monomers for automated RNA synthesis. Switching to the opposite enantiomer of the catalyst allows f

COMPOUNDS FOR TREATING BACTERIAL INFECTIONS

-

, (2011/04/25)

The present invention relates to a novel class of guanine nucleotide analogs which inhibit RelA and Relseq synthetic activity and which possess anti-bacterial activity. The present invention also relates to pharmaceutical compositions that include such compounds, and to methods of use of such compounds or compositions for combating bacteria and treating bacterial infections.

ppGpp analogues inhibit synthetase activity of Rel proteins from Gram-negative and Gram-positive bacteria

Wexselblatt, Ezequiel,Katzhendler, Jehoshua,Saleem-Batcha, Raspudin,Hansen, Guido,Hilgenfeld, Rolf,Glaser, Gad,Vidavski, Roee R.

scheme or table, p. 4485 - 4497 (2010/08/22)

A prominent feature of the stringent response is the accumulation of two unusual phosphorylated derivatives of GTP and GDP (pppGpp: 5′-triphosphate-3′-diphosphate, and ppGpp: 5′-3′-bis-diphosphate), collectively called (p)ppGpp, within a few seconds after the onset of amino-acid starvation. The synthesis of these 'alarmone' compounds is catalyzed by RelA homologues. Other features of the stringent response include inhibition of stable RNA synthesis and modulation of transcription, replication, and translation. (p)ppGpp accumulation is important for virulence induction, differentiation and antibiotic resistance. We have synthesized a group of (p)ppGpp analogues and tested them as competitive inhibitors of Rel proteins in vitro. 2′-Deoxyguanosine-3′-5′-di(methylene bisphosphonate) [compound (10)] was found as an inhibitor that reduces ppGpp formation in both Gram-negative and Gram-positive bacteria. In silico docking together with competitive inhibition analysis suggests that compound (10) inhibits activity of Rel proteins by competing with GTP/GDP for its binding site. As Rel proteins are completely absent in mammalians, this appears to be a very attractive approach for the development of novel antibacterial agents.

Synthesis of 2'-O-substituted ribonucleosides.

Serebryany,Beigelman

, p. 1007 - 1009 (2007/10/03)

An efficient synthesis of 2'-O-substituted ribonucleosides, including 2'-O-TBDMS and 2'-O-TOM protected as well as 2'-O-Me and 2'-O-allyl derivatives is presented. Di-t-butylsilylene group was employed for simultaneous protection of 3'- and 5'- hydroxyl functions of nucleoside on the first step. Subsequent silylation or alkylation of free 2'-OH followed by introduction of suitable protection on the base moiety and removal of cyclic silyl protection gave target compounds in a high yield.

Methods for synthesizing nucleosides, nucleoside derivatives and non-nucleoside derivatives

-

, (2008/06/13)

The present invention provides methods for the chemical synthesis of nucleosides and derivatives thereof, including 2′-amino, 2′-N-phthaloyl, 2′-O-methyl, 2′-O-silyl, 2′OH nucleosides, C-nucleosides, nucleoside phosphoramidites, C-nucleoside phosphoramidites, and non-nucleoside derivatives.

Methods for synthesizing nucleosides, nucleoside derivatives and non-nucleoside derivatives

-

, (2008/06/13)

The present invention provides methods for the chemical synthesis of nucleosides and derivatives thereof, including 2′-amino, 2′-N-phthaloyl, 2′-O-methyl, 2′-O-silyl, 2′-O-triisopropylsilyloxymethyl, 2′-OH nucleosides, C-nucleosides, nucleoside phosphoramidites, C-nucleoside phosphoramidites, and non-nucleoside derivatives.

An efficient preparation of protected ribonucleosides for phosphoramidite RNA synthesis

Serebryany, Vladimir,Beigelman, Leonid

, p. 1983 - 1985 (2007/10/03)

An efficient synthesis of protected ribonucleosides useful for phosphoramidite RNA synthesis is described. Di-t-butylsilylene group was employed for simultaneous protection of 3′- and 5′-hydroxyl functions of nucleoside. Subsequent silylation of free 2′-O

Modified phosphotriester method for chemical synthesis of ribooligonucleotides. Part I. Synthesis of riboundecaadenylate and two fragments constituting the sequence of R-17 translation control signal

Sung, Wing L.,Narang, Saran A.

, p. 111 - 120 (2007/10/02)

A modified phosphotriester method has been succesfully applied for the chemical synthesis of ribooligonucleotides.The starting material is a fully protected ribomononucleoside containing a 3'-phosphotriester group 5.The coupling reaction is performed usin

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