81295-14-9
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Basic information
- Product Name: DPN I
- Synonyms: RESTRICTION ENDONUCLEASES DPN I;DPN I;DPN I RESTRICTION ENZYME;restriction endonuclease dpn I from*diplococcus P;RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE;dpn i from diplococcus pneumoniae;restriction endonuclease Dpn I
- CAS NO:81295-14-9
- Molecular Formula:
- Molecular Weight: 0
- EINECS: N/A
- Product Categories: 3.1.x.x Acting on esters;3.x.x.x Hydrolases;Molecular Biology;Molecular Biology Enzymes;Molecular Biology Tested;Restriction EndonucleasesApplication Index;Restriction EnzymesEnzyme Class Index
- Mol File: 81295-14-9.mol
- Article Data: 0
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Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /buffered aqueous glycerol solution
- Density: N/A
- Refractive Index: N/A
- Storage Temp.: −20°C
- Solubility: N/A
- CAS DataBase Reference: DPN I(CAS DataBase Reference)
- NIST Chemistry Reference: DPN I(81295-14-9)
- EPA Substance Registry System: DPN I(81295-14-9)
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Safety Data
- Hazard Codes: N/A
- Statements: N/A
- Safety Statements: N/A
- RIDADR: UN3373 - class 6.2 Biological substance, Category B
- WGK Germany: 3
- RTECS:
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 81295-14-9(Hazardous Substances Data)
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81295-14-9 Usage
Uses
In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.General Description
IsoschizomersThe enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.Methylation sensitivityThe enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.Typical ligation and recutting assayDpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.Check Digit Verification of cas no
The CAS Registry Mumber 81295-14-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,1,2,9 and 5 respectively; the second part has 2 digits, 1 and 4 respectively.Calculate Digit Verification of CAS Registry Number 81295-14:
(7*8)+(6*1)+(5*2)+(4*9)+(3*5)+(2*1)+(1*4)=129
129 % 10 = 9
So 81295-14-9 is a valid CAS Registry Number.
81295-14-9Upstream product
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DPN I
