Welcome to LookChem.com Sign In|Join Free

CAS

  • or

81295-14-9

DPN I, also known as Dpn I nuclease, is an enzyme that acts as a restriction endonuclease. It is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II, and Sau3A I. DPN I is sensitive to methylation, and its activity is inhibited by the presence of 5-methylcytosine at the indicated site, provided that no 6-methyladenine is present.

81295-14-9 Suppliers

Post Buying Request

Recommended suppliersmore

  • Product
  • FOB Price
  • Min.Order
  • Supply Ability
  • Supplier
  • Contact Supplier
  • 81295-14-9 Structure

  • Basic information

    1. Product Name: DPN I
    2. Synonyms: RESTRICTION ENDONUCLEASES DPN I;DPN I;DPN I RESTRICTION ENZYME;restriction endonuclease dpn I from*diplococcus P;RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE;dpn i from diplococcus pneumoniae;restriction endonuclease Dpn I
    3. CAS NO:81295-14-9
    4. Molecular Formula:
    5. Molecular Weight: 0
    6. EINECS: N/A
    7. Product Categories: 3.1.x.x Acting on esters;3.x.x.x Hydrolases;Molecular Biology;Molecular Biology Enzymes;Molecular Biology Tested;Restriction EndonucleasesApplication Index;Restriction EnzymesEnzyme Class Index
    8. Mol File: 81295-14-9.mol

  • Chemical Properties

    1. Melting Point: N/A
    2. Boiling Point: N/A
    3. Flash Point: N/A
    4. Appearance: /buffered aqueous glycerol solution
    5. Density: N/A
    6. Refractive Index: N/A
    7. Storage Temp.: −20°C
    8. Solubility: N/A
    9. CAS DataBase Reference: DPN I(CAS DataBase Reference)
    10. NIST Chemistry Reference: DPN I(81295-14-9)
    11. EPA Substance Registry System: DPN I(81295-14-9)

  • Safety Data

    1. Hazard Codes: N/A
    2. Statements: N/A
    3. Safety Statements: N/A
    4. RIDADR: UN3373 - class 6.2 Biological substance, Category B
    5. WGK Germany: 3
    6. RTECS:
    7. HazardClass: N/A
    8. PackingGroup: N/A
    9. Hazardous Substances Data: 81295-14-9(Hazardous Substances Data)

81295-14-9 Usage

Uses

Used in PCR-mediated mutagenesis:
DPN I is used as a digestion enzyme for wild type DNA after PCR. This application is crucial for the process of site-directed mutagenesis, where the wild type DNA template is digested, allowing for the incorporation of mutated sequences.
Used in Ligation and Recutting Assays:
In a typical ligation and recutting assay, DPN I fragments obtained from the complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in a buffer containing 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, and 1mM ATP, pH 7.5 (at +20°C). This process allows for the assessment of the percentages of product that can be ligated and subsequently recut with DPN I, yielding the typical pattern of pBR322 × Dpn I fragments.

Check Digit Verification of cas no

The CAS Registry Mumber 81295-14-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,1,2,9 and 5 respectively; the second part has 2 digits, 1 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 81295-14:
(7*8)+(6*1)+(5*2)+(4*9)+(3*5)+(2*1)+(1*4)=129
129 % 10 = 9
So 81295-14-9 is a valid CAS Registry Number.

81295-14-9Upstream product

81295-14-9Downstream Products

Post a RFQ

Enter 15 to 2000 letters.Word count: 0 letters

Attach files(File Format: Jpeg, Jpg, Gif, Png, PDF, PPT, Zip, Rar,Word or Excel Maximum File Size: 3MB)

1

What can I do for you?
Get Best Price

Get Best Price for 81295-14-9