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81295-14-9 Usage


DPN I, also known as Dpn I nuclease, is an enzyme that acts as a restriction endonuclease. It is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II, and Sau3A I. DPN I is sensitive to methylation, and its activity is inhibited by the presence of 5-methylcytosine at the indicated site, provided that no 6-methyladenine is present.


Used in PCR-mediated mutagenesis:
DPN I is used as a digestion enzyme for wild type DNA after PCR. This application is crucial for the process of site-directed mutagenesis, where the wild type DNA template is digested, allowing for the incorporation of mutated sequences.
Used in Ligation and Recutting Assays:
In a typical ligation and recutting assay, DPN I fragments obtained from the complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in a buffer containing 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, and 1mM ATP, pH 7.5 (at +20°C). This process allows for the assessment of the percentages of product that can be ligated and subsequently recut with DPN I, yielding the typical pattern of pBR322 × Dpn I fragments.

Check Digit Verification of cas no

The CAS Registry Mumber 81295-14-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,1,2,9 and 5 respectively; the second part has 2 digits, 1 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 81295-14:
129 % 10 = 9
So 81295-14-9 is a valid CAS Registry Number.

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