82436-78-0

Basic information

• Product Name: N-Hydroxysulfosuccinimide
• Synonyms: 1-Hydroxy-2,5-dioxypyrrolidine-3-sulfonic acid sodium salt;N-Hydroxy-2-(sodiosulfo)succinimide;N-Hydroxysulfosuccinic imide sodium salt;1-Hydroxy-2,5-dioxopyrrolidine-3-sulfonic acid;3-Pyrrolidinesulfonic acid, 1-hydroxy-2,5-dioxo-
• CAS NO: 82436-78-0
• Molecular Formula: C₄H₅NO₆S
• Molecular Weight: 195.15
• Product Categories: proteinmod
• Mol File: 82436-78-0.mol
• Article Data: 1

Chemical Properties

• Appearance: /
• Storage Temp.: 2-8°C
• CAS DataBase Reference: N-Hydroxysulfosuccinimide(CAS DataBase Reference)
• NIST Chemistry Reference: N-Hydroxysulfosuccinimide(82436-78-0)
• EPA Substance Registry System: N-Hydroxysulfosuccinimide(82436-78-0)

Safety Data

• Hazardous Substances Data: 82436-78-0(Hazardous Substances Data)

Usage

General Description

N-Hydroxysulfosuccinimide (NHS) is a water-soluble chemical compound commonly used in the field of bioconjugation and biotechnology. It is a sulfonated derivative of N-hydroxysuccinimide, and its sulfonate group enhances its water solubility and stability. NHS is commonly used as an activating agent in the synthesis of amine-reactive crosslinkers for protein labeling, immobilization of biomolecules, and other bioconjugation applications. Its reactivity with primary amines makes it a valuable tool for the chemical modification of proteins, peptides, and other biomolecules, enabling the attachment of a wide range of functional groups or labels for various biological and biomedical research purposes. The presence of the hydroxyl group in NHS also makes it a useful compound for esterification and amidation reactions. Overall, NHS is a versatile chemical reagent with applications in the fields of chemistry, biology, and medicine.

Downstream Products

• 106145-13-5 N-sulfosuccinimidyl-(4-iodoacetyl)-aminobenzoate 
• 153311-90-1 ε-(p-azidobenzene-sulfonyl)aminocaproic acid (N-hydroxysuccinimide-2-sulfonic acid) ester 

Relevant articles and documents

Labeling reagents useful for the chemical attachment of nitrophenyl derivative ligands to DNA probes

This invention discloses methods and compounds for covalently linking nitro-, dinitro- and trinitrophenyl to DNA and the resulting nitro-, dinitro-, and trinitrophenyl labeled DNA reagents. Reagents for the in situ detection of a chromosome or a region of a chromosome are disclosed. These reagents include a multiplicity of DNA sequences that are complementary to different portions of the chromosome or chromosome region to be detected. Multiple nitro-, dinitro- or trinitrophenyl labels are covalently linked to the DNA sequences by means of an amino acid linking group. These labeled reagents are contacted under hybridizing conditions with the chromosome or chromosome region of interest. Any binding of the reagent with the chromosome or chromosome region of interest may then be detected by immunological techniques.