82989-82-0 Usage
Uses
Used in Microbiology Research:
5-Aminoimidazole-4-carboxamide-1-ribofuranosyl triphosphate is used as a research tool for studying the metabolism of microorganisms. It accumulates in microorganisms when there is a depletion of folate intermediates needed to metabolize ZMP to inosine monophosphate (IMP). This property makes it useful for investigating the mechanisms of nucleotide metabolism and the role of folate in microorganisms.
Used in Drug Development:
5-Aminoimidazole-4-carboxamide-1-ribofuranosyl triphosphate can be used as a starting material for the development of drugs targeting nucleotide metabolism. By understanding its role in the metabolism of microorganisms, researchers can potentially develop drugs that target specific metabolic pathways, leading to the development of new therapeutic agents.
Used in Biochemistry Education:
5-Aminoimidazole-4-carboxamide-1-ribofuranosyl triphosphate can be used as an educational tool in biochemistry courses to teach students about nucleotide metabolism and the role of various intermediates in the synthesis of important biomolecules. This can help students gain a deeper understanding of the complex processes that occur within living organisms.
Check Digit Verification of cas no
The CAS Registry Mumber 82989-82-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,2,9,8 and 9 respectively; the second part has 2 digits, 8 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 82989-82:
(7*8)+(6*2)+(5*9)+(4*8)+(3*9)+(2*8)+(1*2)=190
190 % 10 = 0
So 82989-82-0 is a valid CAS Registry Number.
InChI:InChI=1/C9H17N4O14P3/c10-7-4(8(11)16)12-2-13(7)9-6(15)5(14)3(25-9)1-24-29(20,21)27-30(22,23)26-28(17,18)19/h2-3,5-6,9,14-15H,1,10H2,(H2,11,16)(H,20,21)(H,22,23)(H2,17,18,19)/t3-,5-,6-,9-/m1/s1
82989-82-0Relevant academic research and scientific papers
A Multi-enzyme Cascade for the Biosynthesis of AICA Ribonucleoside Di- and Triphosphate
Eltoukhy, Lobna,Loderer, Christoph
, (2021/12/22)
AICA (5′-aminoimidazole-4-carboxamide) ribonucleotides with different phosphorylation levels are the pharmaceutically active metabolites of AICA nucleoside-based drugs. The chemical synthesis of AICA ribonucleotides with defined phosphorylation is challenging and expensive. In this study, we describe two enzymatic cascades to synthesize AICA derivatives with defined phosphorylation levels from the corresponding nucleobase and the co-substrate phosphoribosyl pyrophosphate. The cascades are composed of an adenine phosphoribosyltransferase from Escherichia coli (EcAPT) and different polyphosphate kinases: polyphosphate kinase from Acinetobacter johnsonii (AjPPK), and polyphosphate kinase from Meiothermus ruber (MrPPK). The role of the EcAPT is to bind the nucleobase to the sugar moiety, while the kinases are responsible for further phosphorylation of the nucleotide to produce the desired phosphorylated AICA ribonucleotide. The selected enzymes were characterized, and conditions were established for two enzymatic cascades. The diphosphorylated AICA ribonucleotide derivative ZDP, synthesized from the cascade EcAPT/AjPPK, was produced with a conversion up to 91 %. The EcAPT/MrPPK cascade yielded ZTP with conversion up to 65 % with ZDP as a side product.
