839709-93-2Relevant academic research and scientific papers
Molecular tripods showing fluorescence enhancement upon binding to streptavidin
Tae, Woo Kim,Hey, Young Yoon,Park, Jung-Hyun,Kwon, Oh-Hoon,Jang, Du-Jeon,Hong, Jong-In
, p. 111 - 114 (2005)
(Chemical Equation Presented) We introduce a new approach to biotin-streptavidin assays based on a molecular tripod which consists of biotin, a fluorophore, and a quencher. The interaction between streptavidin and molecular tripods perturbs the ground-state quencher-fluorophore dimeric conformation in the absence of streptavidin and diminishes the intrinsic self-quenching of a quencher-fluorophore pair. The emission intensity of the molecular tripods plus streptavidin is 3.5-5.2 times that of molecular tripods in the absence of streptavidin.
