852636-01-2Relevant articles and documents
Tuning DNA stability to achieve turnover in template for an enzymatic ligation reaction
Kausar, Abu,McKay, Rosalie D.,Lam, Jade,Bhogal, Rohan S.,Tang, Alexandra Y.,Gibbs-Davis, Julianne M.
, p. 8922 - 8926 (2011/11/06)
Learning to let go: Introducing destabilizing modifications into a DNA template leads to turnover in a DNA-templated ligation reaction. By incorporating a cross-catalytic cycle, self-replication was also achieved, with one target able to make 32 copies of itself (see picture). This destabilization approach represents a general method for incorporating amplification into DNA ligation processes using T4 DNA ligase.