852831-47-1Relevant articles and documents
Microbial hydroxylation of bufalin by Cunninghamella blakesleana and Mucor spinosus
Ye, Min,Han, Jian,Tu, Guangzhong,An, Dongge,Guo, Dean
, p. 626 - 628 (2008/09/21)
The microbial transformation of a cytotoxic bufadienolide, bufalin (1), was carried out using two strains of filamentous fungi. Cunninghamella blakesleana catalyzed the specific 12α-hydroxylation of bufalin and produced 12α-hydroxybufalin (2) and 7β,12α-dihydroxybufalin (3) as the major metabolites, together with 7β-hydroxybufalin (4) and 12β-hydroxybufalin (5) in low yields. Two minor products were isolated from the culture broth of Mucor spinosus and were identified as 7β,15α- dihydroxybufalin (6) and 5β,7β-dihydroxybufalin (7), respectively. Metabolites 2, 3, 6, and 7 are new compounds, and their structures were fully characterized by NMR and MS spectroscopy.