857680-50-3

Upstream product

• 857680-49-0 5-(4-(2,5-dioxo-2H-pyrrol-1⁽⁵⁾-yl)benzamido)isophthalic acid 
• 29305-46-2 4-(2,5-dioxo-2,5-dihydro-1-pyrrol-1-yl)benzoyl chloride 
• 17057-04-4 N-(4-carboxyphenyl)maleimide 
• 150-13-0 4-amino-benzoic acid 

Downstream Products

• 857680-51-4 C₃₅H₂₂N₄O₁₃ 
• 857680-52-5 C₃₅H₁₈Cl₄N₄O₉ 

Relevant academic research and scientific papers

Hemoglobin-superoxide dismutase-chemical linkages that create a dual-function protein

Chemical reagents were designed to cross-link and connect hemoglobin and superoxide dismutase, combining the oxygen transport and superoxide-removal capabilities of the red cell in a dual-function protein. Reaction of 1 with thiol-protected hemoglobin followed by reduction produces cross-linked hemoglobin with a free thiol on the cross-link. Reaction of SOD with 5 produces a cross-linked protein with a maleimide on the cross-link. Addition of the hemoglobin-thiol to the SOD-maleimide produces a protein with the desired dual properties. Hemoglobin's oxygenation cooperativity is lowered as a result of being in the conjugate, while SOD's activity is equal to that of the native protein.