863485-88-5Relevant articles and documents
A highly sensitive fluorescent probe for tracking intracellular zinc ions and direct imaging of prostatic tissue in mice
Wang, Yuyu,Duan, Houna,Shi, Hongyuan,Zhang, Shiwei,Xu, Yufang,Zhu, Weiping,Qian, Xuhong
, p. 2933 - 2936 (2020)
A highly sensitive fluorescent sensor ZnDN was designed, synthesized and used for tracking intracellular zinc ions in various living cells and direct imaging of prostatic tissue in mice. ZnDN was prepared from the heterocyclic-fused naphthalimide fluorophore, and the zinc receptor, N,N-bis(2-pyridylmethyl)ethylenediamine (BPEN). Upon addition of Zn2+ to the solutions of ZnDN, a remarkable fluorescence enhancement was observed, which could be attributed to the photo-induced electron transfer (PET) mechanism. Since ZnDN exhibited high sensitivity toward Zn2+ in phosphate buffer solution, with a limit of detection of 4.0 × 10?9 mol/L, it was further applied for the imaging of exogenous and endogenous Zn2+ in different living cells. Living cells imaging experiments suggested that ZnDN could image the changes of intracellular free zinc ions, and could be used for two-photon imaging. Moreover, flow cytometry suggested that ZnDN could distinguish cancerous prostate cells from normal cells. Animal experiments indicated that ZnDN had the potential in imaging prostate tissue in vivo.
A lysosomal targeting fluorescent probe and its zinc imaging in SH-SY5Y human neuroblastoma cells
Duan, Houna,Ding, Yu,Huang, Chusen,Zhu, Weiping,Wang, Rui,Xu, Yufang
supporting information, p. 55 - 57 (2018/04/05)
Zinc plays a significant role in oxidative balance and central nervous systems. Herein, we reported a highly sensitive fluorescent probe DR, bearing a morpholine group and a BPEN ligand in the naphthalimide fluorophore. Upon Zn2+ binding, DR ex
Nickel(ii) and iron(iii) selective off-on-type fluorescence probes based on perylene tetracarboxylic diimide
Wang, Haixia,Wang, Delou,Wang, Qi,Li, Xiyou,Schalley, Christoph A.
scheme or table, p. 1017 - 1026 (2010/05/19)
Two novel "turn-on" fluorescent probes with perylene tetracarboxylic diimide (PDI) as the fluorophore and two different di-(2-picolyl)-amine (DPA) groups as the metal ion receptor (PDI-1 and PDI-2) were successfully synthesized with satisfactory yields. PDI-1 exhibited high selectivity toward Ni2+ in the presence of various other metal cations including Zn2+, Cd2+ and Cu2+ which were expected to interfere significantly. A 1:2 stoichiometry was found for the complex formed by PDI-1 and Ni2+ by a Job's plot and by non-linear least square fitting of the fluorescence titration curves. By introducing an extra diamino ethylene group between DPA and the phenyl bridge, the receptor was modified and the high selectivity of the sensor toward Ni2+ shifted to Fe3+. The enhancement factor of the fluorescence response of PDI-2 to Fe3+ was as high as 138. The binding behavior of the receptors in these two compounds is affected significantly by the PDI fluorophores. Most interestingly, both Ni2+ and Fe3+ are paramagnetic metal ions, which are known as fluorescence quenchers and are rarely targeted with "turn-on" fluorescence probes. This result suggests that PDIs are favorable fluorophores for a "turn-on" fluorescence probe for paramagnetic transition metal ions because of their high oxidation potential. The Royal Society of Chemistry.
A pH-resistant Zn(II) sensor derived from 4-aminonaphthalimide: Design, synthesis and intracellular applications
Wang, Jiaobing,Xiao, Yi,Zhang, Zhichao,Qian, Xuhong,Yang, Yuanyuan,Xu, Qin
, p. 2836 - 2839 (2007/10/03)
The development and intracellular applications of a water-soluble Zn(II) sensor (WZS) are described. 4-Aminonaphthalimide is used as the fluorophore of WZS for its high photostability, long excitation and emission wavelength, large Stokes' shift and inert response to pH. N,N-Bis(2-pyridylmethyl)ethylenediamine (BPEN), used as a receptor for Zn(II), is attached to the fluorophore through a rigid benzene framework on the imide moiety. Spectroscopic studies indicate that this fluorophore-receptor linking strategy could be used for the development of effective photoinduced electron transfer (PET) sensors. WZS is pH-insensitive and shows high selectivity for Zn(II) against other biologically relevant metal ions. Cellular applications revealed that WZS could be used for the imaging of intracellular Zn(II). The Royal Society of Chemistry 2005.
