867020-31-3Relevant academic research and scientific papers
Highly sensitive and selective strategy for imaging Hg2+ using near-infrared squaraine dye in live cells and zebrafish
Wang, Guimei,Xu, Wenjian,Yang, Huanghao,Fu, Nanyan
, p. 369 - 376 (2018)
This article describes the rational design and synthesis of a monothiosquaraine (MTSQ) fluorescent Hg2+ probe that exhibits near-infrared fluorescence, ultrahigh brightness, fast response, high sensitivity and excellent selectivity. Utilizing the strategy of the incorporation of the heavy atom effect and self-assembly caused quenching, the background fluorescence of MTSQ was greatly lowered and the sensitivity in PBS buffer solution was highly enhanced with a very low limit of detection (LOD = 1.2 nM). Systematic studies revealed excellent selectivity and specificity for Hg2+ over other thiophilic metal ions such as Ag+, Pb2+, and Cd2+. The mechanistic investigation indicates that self-assembly and desulfurization of MTSQ are responsible for Hg2+-triggered significantly higher fluorescence enhancement. With high sensitivity, optimal pH range of 3–7, weak background, good water-solubility and low cytotoxicity, MTSQ therefore can be applied for imaging Hg2+ in live cells and zebrafish. The very simple but effective strategy reported here can provide a more convenient approach for designing NIR probes.
Water-soluble near-infrared monothio-squaric acid dye as well as preparation method and application thereof
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Paragraph 0029, (2017/08/29)
The invention discloses water-soluble near-infrared monothio-squaric acid dye as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing oxo-squaric acid dye and a Lawesson's reagent; dissolvi
An asymmetric near red foreign acid dye and its preparation method and application
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Paragraph 0032; 0033; 0034, (2018/02/04)
The invention discloses an asymmetric near-infrared squaraine dye, a preparation method thereof and an application thereof. According to the invention, an m-aminophenol derivative and the squaric acid are mixed to dissolve in an appropriate solvent, and then are subjected to the backflow water-diversion treatment in the nitrogen protection environment. After that, the resultant obtained reaction mixture is cooled to be at the room temperature and subjected to the decompression treatment to remove the solvent. The resultant crude product is purified through the silica gel column chromatography process to prepare the asymmetric near-infrared squaraine dye. According to the technical scheme of the invention, through introducing an oxygen-ether chain, the dissolvability of the squaraine dye in an aqueous solution can be effectively improved. The obtained asymmetric near-infrared squaraine dye is good in stability and excellent in optical performance, which can be used as a protein biomarker for dual responses of fluorescence reaction and colorimetric reaction. The asymmetric near-infrared squaraine dye can be applied in multiple fields of biological molecule marking, analyzing, separating, and the like.
Fluorescent Dyes and Complexes
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Page/Page column 8, (2008/06/13)
A fluorescent dye comprising a xanthene-derived fluorophore having the formula (I) wherein R1, R2, R3, R4, R5 and R6 are independently selected from H, alkyl, alkoxy, alcohol, ether, alkenyl, alkenoxy, aryl, alkaryl, aralkyl and amido, except that R1, R4 and/or R5 is not H when bonded to Y, Y1 or Y2, respectively; X is either O? or S?; and at least one of Y, Y1, Y2, Y3, Y4 and Y5 is a group for covalently bonding the dye, optionally through. the use of a coupling agent, to a target molecule, and is otherwise H. The dye may be covalently attached to a target molecule to form a complex, and the dye and/or complex finds use in cell analysis techniques, particularly pH measurement and analysis of kinetics of migration.
