872003-30-0Relevant academic research and scientific papers
Chemical primer extension: Efficiently determining single nucleotides in DNA
Hagenbuch, Patrizia,Kervio, Eric,Hochgesand, Annette,Plutowski, Ulrich,Richert, Clemens
, p. 6588 - 6592 (2005)
Rapid replication: Non-enzymatic primer extension has previously been studied in the context of prebiotic chemistry, but not for practical applications. Reactions with primers featuring a 3′-amino-2′, 3′-dideoxy-nucleotide can be rapid and selective for all four templating nucleobases (see scheme). On a chip with immobilized capture strands, 500 fmol of template suffice for single-nucleotide determinations within 2.7 h.
POLYMERASE-INDEPENDENT ANALYSIS OF THE SEQUENCE OF POLYNUCLEOTIDES
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Page/Page column 42, (2008/06/13)
The present invention concerns methods of polymerase independent template directed elongation of polynucleotides, nucleotide building blocks used in these methods as well as the use of the methods and building blocks for the determination of nucleotide sequences, in particular for the determination of SNPs, base modifications, mutations, rearrangements and methylation patterns.
