87403-37-0Relevant academic research and scientific papers
Enzymatic Formation of a New Monosaccharide, 1-Deoxy-D-altro-heptulose Phosphate, from DL-Acetoin and D-Ribose 5-Phosphate by a Transketolase Mutant of Bacillus pumilus
Yokota, Akira,Sasajima, Ken-ichi
, p. 1545 - 1553 (2007/10/02)
1-Deoxy-D-altro-heptulose phosphate (DAHP) synthase activity was found in the cell-free extract of a transketolase mutant, BG2532, of Bacillus pumilus IFO 12089.The enzyme was partially purified by protamine sulfate treatment, ammonium sulfate fractionation, DEAE-cellulose and hydroxylapatite column chromatography.When DL-acetoin and D-ribose 5-phosphate were incubated with the partially purified enzyme preparation, DAHP and acetaldehyde were detected as reaction products.Thiamine pyrophosphate (TPP) and Mg(2+) were required as cofactors.The results of the stoichiometric measurements indicate that DAHP synthesis proceeds according to the following formula: .Intracellular DAHP formed by this reaction may be excreted and accumulated in culture broth as 1-deoxy-D-altro-heptulose after dephosphorylation.
