88200-89-9Relevant academic research and scientific papers
1H, 2H, 19F, 14N ENDOR and TRIPLE Resonance Investigations of Substituted Flavin Radicals in Their Different Protonation States
Weilbacher, E.,Helle, N.,Elsner, M.,Kurreck, H.,Mueller, F.,Allendoerfer, R. D.
, p. 64 - 72 (2007/10/02)
A variety of isotopically labelled and/or substituted flavins have been converted into their corresponding radical states.Cation and neutral radicals were generated chemically and anion radicals were obtained electrochemically.By performing ENDOR and TRIPLE resonance experiments, complete sets of hyperfine coupling constants including their signs were accessible.The hyperfine data allowed (a) identification of the radical state present, (b) information to be obtained about the preferred conformational arrangements of the substituents and (c) conclusions to be drawn about the influences of the substituents on the spin density distributions of the different radical states. - KEY WORDS: ENDOR, TRIPLE resonance spectroscopy; Flavin radicals; Electrochemical generation of radicals.
Fluid Solution and Solid-State Electron Nuclear Double Resonance Studies of Flavin Model Compounds and Flavoenzymes
Kurreck, H.,Bock, M.,Bretz, N.,Elsner, M.,Kraus, H.,et al.
, p. 737 - 746 (2007/10/02)
Partially deuterated and various substituted flavin and thiaflavin model compounds have been synthesized.For the first time, high-resolution H, D, and 14N ENDOR and TRIPLE resonance experiments in fluid solution have been performed on the paramagnetic derivatives of these compounds.Additionally, valuable information has been obtained about hyperfine anisotropies and molecular structures from ENDOR in rigid matrices.Solid matrix ENDOR studies of native flavoenzymes, namely, "Old Yellow Enzyme" (NADPH dehydrogenase), two flavodoxins, and a methanol oxidase are reported.The ENDOR matrix signals of the various flavoproteins are different in intensity, suggesting that the microenvironments are remarkably different.Applicabilities and limitations of the ENDOR technique in the studies of flavins and flavoenzymes are discussed.
