88247-69-2 Usage
Uses
Used in Pharmaceutical Research:
25-Hydroxy Cholesterol-d6 is used as a research tool for studying the mechanisms of human immunodeficiency virus replication and the induction of apoptosis in human monocytic cell lines. Its labeled nature allows for accurate tracking and measurement in experimental settings.
Used in Virology:
In the field of virology, 25-Hydroxy Cholesterol-d6 is used as an inhibitor of human immunodeficiency virus replication, providing insights into the development of potential antiviral therapies.
Used in Cell Biology:
25-Hydroxy Cholesterol-d6 is used as a tool in cell biology to study the induction of apoptosis in CEM cells and its association with the negative regulation of c-Myc, contributing to a better understanding of cell death mechanisms and their regulation.
Biochem/physiol Actions
25-hydroxycholesterol inhibits cholesterol biosynthesis by downregulating the function of 3-hydroxy-3-methylglutaryl-CoA reductase. It is found to be implicated in atherosclerosis. 25-hydroxycholesterol levels are known to be increased in hypercholesterolemic condition.
Check Digit Verification of cas no
The CAS Registry Mumber 88247-69-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,8,2,4 and 7 respectively; the second part has 2 digits, 6 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 88247-69:
(7*8)+(6*8)+(5*2)+(4*4)+(3*7)+(2*6)+(1*9)=172
172 % 10 = 2
So 88247-69-2 is a valid CAS Registry Number.
88247-69-2Relevant academic research and scientific papers
Synthesis of side-chain oxysterols and their enantiomers through cross-metathesis reactions of Δ22 steroids
Brownholland, David P.,Covey, Douglas F.
, p. 22 - 31 (2017/03/24)
A synthetic route that utilizes a cross-metathesis reaction with Δ22 steroids has been developed to prepare sterols with varying C-27 side-chains. Natural sterols containing hydroxyl groups at the 25 and (25R)-26 positions were prepared. Enantiomers of cholesterol and (3β,25R)-26-hydroxycholesterol (27-hydroxycholesterol) trideuterated at C-19 were prepared for future biological studies.
Synthesis of Cholesterol and Derivatives substituted in the Side chain
Kirk, David N.,Varley, Michael J.,Makin, Hugh L.J.,Trafford, David J.H.
, p. 2563 - 2567 (2007/10/02)
The carbanion derived from 24-phenylsulphonylchol-5-en-3β-ol 3-tetrahydropyranyl ether (6) reacted with acetone to give the 24-phenylsulphonyl-25-hydroxycholesterol derivative (7a), which was reduced by sodium amalgam to a separable mixture of the labelled 25-hydroxycholesterol and cholest-5,24-dien-3β-ol (desmosterol) derivatives. Cholesterol has been obtained via a selective reduction of the Δ24-unsaturation in desmosteryl benzoate with di-imide, or more efficiently by reducing 25-hydroxycholesteryl 3,25-diacetate w ith litium in ethylamine, without significant loss of label.The labelled 24,25-dihydroxycholesterols were also prepared from desmosteryl benzoate.
