88261-81-8Relevant academic research and scientific papers
LYSOSOMAL-ENZYME TARGETING: THE PHOSPHORYLATION OF SYNTHETIC D-MANNOSYL SACCHARIDES BY UDP-N-ACETYLGLUCOSAMINE:LYSOSOMAL-ENZYME N-ACETYLGLUCOSAMINE-PHOSPHOTRANSFERASE FROM RAT-LIVER MICROSOMES AND FIBROBLASTS
Madiyalakan, Ragupathy,Chowdhary, Manjit S.,Rana, Surjit S.,Matta, Khushi L.
, p. 183 - 194 (1986)
Phosphorylation of the D-mannose residues of lysosomal-enzymes is essential for the uptake and intracellular transport of these enzymes to lysosomes.The GlcNAc-P-transferase which is involved in the phosphorylation reaction seems to recognize a signal, probably a protein conformation, common to many lysosomal enzymes.To evaluate the role of the carbohydrate portion of the enzyme in these phosphorylation reactions, the acceptor specificity of GlcNAc-P-transferase from rat-liver microsomes and fibroblasts was examined with the aid of synthetic D-mannosyl disaccharides and derivatives that are closely related to the high-mannose type of oligosaccharides.Four methyl D-mannobiosides were synthesized, and their structures were established by 13C-n.m.r. spectroscopy.Of all the D-mannosyl saccharides tested, α-D-Man-(1->2)-α-D-Man-(1->OMe) was found to be the best acceptor, thereby suggesting that oligosaccharide structure may also have a role to play in recognition by this enzyme.
A SYNTHESIS OF 3-O-(α-D-MANNOPYRANOSYL)-D-MANNOSE AND ITS PROTEIN CONJUGATE
Awad, Laila Fathy,El Ashry, El Sayed H.,Schuerch, Conrad
, p. 69 - 80 (2007/10/02)
Methods for the synthesis of 3-O-(α-D-mannopyranosyl)-D-mannose and 2-(4-aminophenyl)ethyl 3-O-(α-D-mannopyranosyl)-α-D-mannopyranoside have been investigated by a number of sequences.Glycosidations with 2,3-di-O-acetyl-4,6-di-O-benzyl-D-mannopyranosyl an
