885684-75-3Relevant academic research and scientific papers
New quinoline-based caging groups synthesized for photo-regulation of aptamer activity
Li, Yi Ming,Shi, Jing,Cai, Rong,Chen, XiaoYun,Luo, Zhao Feng,Guo, Qing Xiang
, p. 129 - 134 (2010)
A series of quinoline-based photo-removable protecting groups for photo-regulation of thrombin aptamer (HD1) activity were synthesized with improved caging and uncaging efficiency. Among them, 8-bromo-2-diazomethyl-7-hydroxyquinolinyl (BHQ-diazo, 1) chromophore was found to cage the HD1 with highest caging and restoration efficiency. Moreover, on the basis of the RP-HPLC and SPR analysis, BHQ was demonstrated to regulate HD1s specific affinity to target molecule with 3-fold photolysis sensitivity and about 40% percent higher uncaging efficiency than Bhc (6-bromo-7-hydroxycoumarin-4-ylmethyl) group. It was proposed that the development and use of quinoline derivative may provide a general strategy to photo-regulate oligonucleotide's activity with improved caging and uncaging efficiencies by the convenient non-site-specific caging method.
Photoregulation of protein plasmid expression in vitro and in vivo using BHQ caging group
Zhang, Zhi Ping,Li, Yi Ming,Chen, Xiao Yun,Guo, Qing Xiang
, p. 338 - 341 (2011)
Green fluorescent protein (GFP) plasmid was caged by 8-bromo-7- hydroxyquinolinyl chromophore (BHQ) for controlling its expression with exact spatiotemporal resolution. In vitro and in vivo experiments clearly verified that, comparing with Bhc caging, the
8-Bromo-7-hydroxyquinoline as a photoremovable protecting group for physiological use: Mechanism and scope
Zhu, Yue,Pavlos, Christopher M.,Toscano, John P.,Dore, Timothy M.
, p. 4267 - 4276 (2007/10/03)
Two-photon excitation (2PE) of "caged" biomolecules represents a powerful method to investigate the temporal and spatial relevance of physiological function in real time and on living tissue, because the excitation volume can be restricted to 1 fL. Additi
