897929-08-7

Basic information

• Product Name: 3'-amino-6'-(tert-butoxycarbonylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
• Synonyms: 3'-amino-6'-(tert-butoxycarbonylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
• CAS NO: 897929-08-7
• Molecular Weight: 430.46
• Mol File: 897929-08-7.mol

Chemical Properties

• CAS DataBase Reference: 3'-amino-6'-(tert-butoxycarbonylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one(CAS DataBase Reference)
• NIST Chemistry Reference: 3'-amino-6'-(tert-butoxycarbonylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one(897929-08-7)
• EPA Substance Registry System: 3'-amino-6'-(tert-butoxycarbonylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one(897929-08-7)

Safety Data

• Hazardous Substances Data: 897929-08-7(Hazardous Substances Data)

Upstream product

• 509-72-8 3',6'-diamino-spiro[phthalan-1,9'-xanthen]-3-one 
• 24608-52-4 tert-butyl chloroformate 
• 24424-99-5 di-tert-butyl dicarbonate 

Downstream Products

• 1346550-28-4 C₃₅H₂₈N₄O₁₀ 

Relevant articles and documents

A caged, localizable rhodamine derivative for superresolution microscopy

A caged rhodamine 110 derivative for the specific labeling of SNAP-tag fusion proteins is introduced. The caged rhodamine 110 derivative permits the labeling of cell surface proteins in living cells and of intracellular proteins in fixed cells. The probe requires only a single caging group to maintain the fluorophore in a non-fluorescent state and becomes highly fluorescent after uncaging. The high contrast ratio is confirmed both in bulk and at the single molecule level. This property, together with its high photon yield makes it an excellent dye for photoactivated localization microscopy (PALM), as we demonstrate here.

Reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells

Hydrogen sulfide (H2S) is emerging as an important mediator of human physiology and pathology but remains difficult to study, in large part because of the lack of methods for selective monitoring of this small signaling molecule in live biological specimens. We now report a pair of new reaction-based fluorescent probes for selective imaging of H2S in living cells that exploit the H2S-mediated reduction of azides to fluorescent amines. Sulfidefluor-1 (SF1) and Sulfidefluor-2 (SF2) respond to H2S by a turn-on fluorescence signal enhancement and display high selectivity for H2S over other biologically relevant reactive sulfur, oxygen, and nitrogen species. In addition, SF1 and SF2 can be used to detect H2S in both water and live cells, providing a potentially powerful approach for probing H2S chemistry in biological systems.

FLUORESCENT MOLECULE

It is an object of the present invention to provide an on-off type fluorescent compound used in gene analyses, which is highly stable and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a compound represented by the following formula (1) or (2): wherein R1 represents an alkyl group containing 1 to 6 carbon atoms, an amino group, an amide group optionally having a protecting group or a substituent, a halogen atom, or a hydrogen atom; R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom; R3 represents an alkyl group containing 1 to 6 carbon atoms, an aryl group, or a hydrogen atom; R4 represents a group containing an oxygen atom, or a hydrogen atom, wherein R3 may bind to R4 to form a ring.