89797-68-2 Usage
Chemical Properties
White crystals
Application
5-(Ethylthio)-1H-tetrazole is used a powerful activator in DNA and RNA syntheses.
Preparation
5-(Ethylthio)-1H-Tetrazole is prepared from the corresponding alkyl thiocyanate and sodium azide through a [2?+?3] cycloaddition under phase transfer catalytic conditions (eq 1). The ratio of the solvent (water to toluene) as well as the reaction temperature are important factors in obtaining preparative yields of the alkylthiotetrazoles. 5-Ethylthio-1H-tetrazole has also been prepared using DMF as solvent albeit in much lower yield.
General Description
5-(Ethylthio)-1H-tetrazole (ETT) is an efficient activator, that activates nucleoside phosphoramidites towards condensation with a nucleoside to form dinucleoside phosphates during oligonucleotide synthesis.
Purification Methods
recrystallized from toluene.
Check Digit Verification of cas no
The CAS Registry Mumber 89797-68-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,9,7,9 and 7 respectively; the second part has 2 digits, 6 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 89797-68:
(7*8)+(6*9)+(5*7)+(4*9)+(3*7)+(2*6)+(1*8)=222
222 % 10 = 2
So 89797-68-2 is a valid CAS Registry Number.
InChI:InChI=1/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)
