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Preparation method and application of iprodione hapten and antigen
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Paragraph 0022, (2019/02/17)
The invention provides a preparation method and application of iprodione hapten and antigen. The iprodione hapten is obtained through the following steps: taking 3,5-dichlorophenyl isocyanate and ethyl glycinate hydrochloride to react to generate ethyl 2-(3-(1,5-dichlorophenylcarbamido)) acetate, hydrolyzing to obtain 2-(3-(1,5-dichlorophenylcarbamido))acetic acid, carrying out ring-forming reaction to obtain 3-(3,5-dichlorophenyl)-2,4-imidazolidindione, reacting with methyl 6-isocyanatocaproate generated by reaction of methyl 6-aminohexanoate hydrochloride and triphosgene, so as to generate methyl 6-(3-(3,5-dichlorophenyl)-2,4-dioxyiminazolealkyl-1-formylamino)caproate; finally, hydrolyzing under an acidic condition. The iprodione antigen is obtained by coupling the iprodione hapten and carrier protein. The antigen prepared by the invention has a specific iprodione antigenic determinant, so that a high-specificity iprodione monoclonal antibody is possibly screened. A generated antibody has high specificity and high sensitivity and can be used for enzyme-linked immunization and rapid determination of test paper.
