62584-33-2

Usage

InChI:InChI=1/C8H6Cl2N2O3/c9-4-1-5(10)3-6(2-4)11-7(13)12-8(14)15/h1-3H,(H,14,15)(H2,11,12,13)/p-1

Upstream product

• 27387-87-7 3-(3',5'-Dichlorophenyl)imidazolidine-2,4-dione 
• 36734-19-7 Iprodione 
• 34893-92-0 3,4-dichlorophenyl isocyanate 
• 56-40-6 glycine 
• 626-43-7 3,5-Dichloroaniline 
• 904752-31-4 ethyl 2-(3-(1,5-dichlorophenylureido))acetate 

Downstream Products

• 626-43-7 3,5-Dichloroaniline 
• 36734-17-5 3-(3,5-dichloro-phenyl)-2,4-dioxo-imidazolidine-1-carboxylic acid ethylamide 
• 36734-19-7 Iprodione 
• 27387-87-7 3-(3',5'-Dichlorophenyl)imidazolidine-2,4-dione 
• 36734-13-1 3-(3,5-dichloro-phenyl)-2,4-dioxo-imidazolidine-1-carboxylic acid propylamide 

Relevant academic research and scientific papers

Preparation method and application of iprodione hapten and antigen

The invention provides a preparation method and application of iprodione hapten and antigen. The iprodione hapten is obtained through the following steps: taking 3,5-dichlorophenyl isocyanate and ethyl glycinate hydrochloride to react to generate ethyl 2-(3-(1,5-dichlorophenylcarbamido)) acetate, hydrolyzing to obtain 2-(3-(1,5-dichlorophenylcarbamido))acetic acid, carrying out ring-forming reaction to obtain 3-(3,5-dichlorophenyl)-2,4-imidazolidindione, reacting with methyl 6-isocyanatocaproate generated by reaction of methyl 6-aminohexanoate hydrochloride and triphosgene, so as to generate methyl 6-(3-(3,5-dichlorophenyl)-2,4-dioxyiminazolealkyl-1-formylamino)caproate; finally, hydrolyzing under an acidic condition. The iprodione antigen is obtained by coupling the iprodione hapten and carrier protein. The antigen prepared by the invention has a specific iprodione antigenic determinant, so that a high-specificity iprodione monoclonal antibody is possibly screened. A generated antibody has high specificity and high sensitivity and can be used for enzyme-linked immunization and rapid determination of test paper.

Preparation of 3 - (3,5-dichlorophenyl) - 2,4-imidazolidinedione method

The invention discloses a method for preparing 3-(3, 5-dichlorophenyl)-2, 4-imidazolidinedione with a structure as shown in a formula (I). The method comprises the following steps: (1) contacting one or a plurality of compounds shown by a formula (III) with compounds shown by a formula (II) under condensation reaction conditions in the presence of a condensation reaction catalyst and an organic solvent to obtain a mixture containing compounds shown by a formula (IV); and (2) directly contacting the mixture containing compounds shown by the formula (IV) with a ring closing reaction catalyst or separating out the compounds shown by the formula (IV) and contacting the compounds with the ring closing reaction catalyst under ring closing reaction conditions. The reaction yield of the method is high, and the intermediate does not need to be separated or purified, so that the steps are simple.

Iprodione degradation by isolated soil microorganisms

Three bacterial strains were isolated from soils adapted to iprodione and identified as Pseudomonas fluorescens, Pseudomonas sp. and Pseudomonas paucimobilis. The first two strains transformed iprodione to N-(3,5- dichlorophenyl)-2,4-dioxoimidazo-lidine (II) and under restrictive conditions to 3,5-dichlorophenylurea acetic acid (III); the latter subsequently degraded II to III and III to 3,5-dichloroaniline (3,5-D). We constructed bacterial combinations consisting of Pseudomonas paucimobilis plus one of the iprodione degraders and showed that these combinations transformed iprodione into 3,5- D. It is known that 3,5-D was the major metabolite found in adapted soils, suggesting that such a bacterial combination might be responsible for degrading iprodione into 3,5-D in adapted soils. Plasmids could only be isolated in Pseudomonas fluorescens but we did not investigate if one of these was involved in the ability to degrade iprodione.