913645-93-9

Upstream product

• 87865-78-9 3',5'-O-(1,1,3,3-tetraisopropyldisiloxan-1,3-diyl)-N₂-isobutyrylguanosine 
• 139434-70-1 N-isobutyryl-3′,5′-O-(1,1,3,3-tetraisopropyldisiloxane-1,3-diyl)-2′-O-[(methylthio)methyl]guanosine 
• 913645-92-8 N²-isobutyryl-3',5'-O-(1,1,3,3-tetraisopropyldisiloxane-1,3-diyl)-2'-O-(tert-butyldithiomethyl)guanosine 
• 64350-24-9 N²-isobutyryl-2'-deoxyguanosine 

Downstream Products

• 913645-94-0 N²-isobutyryl-O⁶-diphenylcarbamoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldithiomethyl)guanosine 

Relevant academic research and scientific papers

Synthesis of RNA using 2′-O-DTM protection

tert-Butyldithiomethyl (DTM), a novel hydroxyl protecting group, cleavable under reductive conditions, was developed and applied for the protection of 2′-OH during solid-phase RNA synthesis. This function is compatible with all standard protecting groups used in oligonucleotide synthesis, and allows for fast and high-yield synthesis of RNA. Oligonucleotides containing the 2′-O-DTM groups can be easily deprotected under the mildest possible aqueous and homogeneous conditions. The preserved 5′-O-DMTr function can be used for high-throughput cartridge RNA purification. Copyright