920975-58-2 Usage
Uses
Used in Biochemical Research:
4-Methylumbelliferyl 2,3,4-Tri-O-benzoyl-6-O-(tert-butyldiphenylsilyl)-β-D-galactopyranoside is used as an intermediate for the production of fluorescent indicators in biochemical research. Its role is crucial for the development of sensitive and specific assays that can detect and measure the activity of certain enzymes, particularly sulphatases.
Used in Enzyme Activity Assays:
In the field of enzyme activity assays, 4-Methylumbelliferyl 2,3,4-Tri-O-benzoyl-6-O-(tert-butyldiphenylsilyl)-β-D-galactopyranoside is utilized as a substrate to determine sulphatase activity. 4-Methylumbelliferyl 2,3,4-Tri-O-benzoyl-6-O-(tert-butyldiphenylsilyl)-β-D-galactopyranoside's structure allows for the release of a fluorescent product upon enzymatic action, which can be easily quantified using fluorescence detection methods. This makes it a valuable tool for studying the function and regulation of sulphatases, as well as for diagnostic and therapeutic applications related to these enzymes.
Used in Pharmaceutical Development:
4-Methylumbelliferyl 2,3,4-Tri-O-benzoyl-6-O-(tert-butyldiphenylsilyl)-β-D-galactopyranoside may also find application in the pharmaceutical industry, particularly in the development of drugs targeting sulphatase enzymes. By understanding the interactions between 4-Methylumbelliferyl 2,3,4-Tri-O-benzoyl-6-O-(tert-butyldiphenylsilyl)-β-D-galactopyranoside and sulphatases, researchers can gain insights into the design of potential therapeutic agents that modulate the activity of these enzymes, which may have implications for the treatment of various diseases.
Check Digit Verification of cas no
The CAS Registry Mumber 920975-58-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 9,2,0,9,7 and 5 respectively; the second part has 2 digits, 5 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 920975-58:
(8*9)+(7*2)+(6*0)+(5*9)+(4*7)+(3*5)+(2*5)+(1*8)=192
192 % 10 = 2
So 920975-58-2 is a valid CAS Registry Number.
920975-58-2Relevant academic research and scientific papers
Indurugalla, Deepani,Watson, Jacqueline N.,Bennet, Andrew J.
, p. 4453 - 4459 (2006)
Two isomeric 4-methylumbelliferyl-α-d-N- acetylneuraminylgalactopyranosides (1 and 2) were synthesised. These compounds contain either the natural α-2,3 or α-2,6 sialyl-galactosyl linkages, as well as an attached 4-methylumbelliferone for convenient detection of their hydrolyses. These compounds were designed as natural sialoside analogues to be used in a continuous assay of sialidase activity, where the sialidase-catalysed reaction is coupled with an exo-β-galactosidase- catalysed hydrolysis of the released galactoside to give free 4-methylumbelliferone. The kinetic parameters for 1 and 2 were measured using the wild-type and nucleophilic mutant Y370G recombinant sialidase from Micromonospora viridifaciens. Kinetic parameters for these analogues measured using the new continuous assay were in good agreement with the parameters for the natural substrate, 3′-sialyl lactose. Given the selection of commercially available exo-β-galactosidases that possess a variety of pH optima, this new method was used to characterise the full pH profile of the wild-type sialidase with the natural sialoside analogue 1. Thus, use of these new substrates 1 and 2 in a continuous assay mode, which can be detected by UV/Vis or fluorescence spectroscopy, makes characterisation of sialidase activity with natural sialoside linkages much more facile. The Royal Society of Chemistry.
