92557-55-6Relevant academic research and scientific papers
Role of the stereochemistry of 3'-fluoro-3'-deoxy analogues of 2-5A in binding to and activation of mouse RNase L
Kalinichenko, Elena N.,Podkopaeva, Tatjana L.,Poopeiko, Nicolai E.,Kelve, Merike,Saarma, Mart,et al.
, p. 43 - 50 (2007/10/02)
The synthesis of two sets of analogues of 2-5A trimer containing 9-(3-fluoro-3-deoxy-β-D-xylo-furanosyl)adenine (AF) or 3'-fluoro-3'-deoxyadenosine (AF) at different positions of the chain is described, along with the preparation of the corresponding 5'-monophosphates and 5'-diphosphorylated (core) trimers.The ability of each ribo and xylo isomeric pair of fluorodeoxy analogues of 2-5A (i) to compete with p3(A2'p)3A3'pC3'p for binding to RNase L in L929 cell extracts, and (ii) to activate the partially purified RNase L from L929 cell extracts to hydrolyze poly(U), was compared to that of the related 3'-deoxy analogue and the parent trimer, p3A3, using radiobinding and RNase L-(2',5')pentaadenylate(core)-agarose assays, respectively.Evidence is presented to show that the stereochemistry of the trimers plays an important role, specifically in the second process.The most striking observation is that, compared to 2-5A, p3A(AF)A was found to be nine times more effective an activator of RNase L, whereas isomeric p3A(AF)A is 30 times less effective.
Nucleotides XXIV; Preparative Synthesis of Trimeric (2'-5')Oligoadenylic Acid
Kvasyuk, Evgeny I.,Kulak, Tamara I.,Khripach, Natalya B.,Mikhailopulo, Igor A.,Uhlmann, Eugen,et al.
, p. 535 - 541 (2007/10/02)
A preparative synthesis of the 2'-5'-Oligoadenylate trimer 19 via the phosphotriester approach is described.Selectively benzoylated derivatives of adenosine were used as starting materials.
SYNTHESIS OF A MODIFIED 2',5'-ADENYLATE TRIMER WITH A 2',3'-DI-O-(CARBOXYETHYL)-ETHYLIDENE TERMINAL GROUP
Kvasyuk, Evgeny I.,Kulak, Tamara I.,Zaitseva, Galina T.,Mikhailopulo, Igor A.,Charubala, Ramamurthy,Pfleiderer, Wolfgang
, p. 3683 - 3686 (2007/10/02)
The trimer of 2',5'-oligoadenylic acid with a (2-carboxyethyl)athylidene group (16) at the 2'-terminal adenosine moiety and its 3'-deoxyadenosine analog (17) have been synthesized by the phosphotriester method.
