93712-94-8Relevant articles and documents
Myo-inositol Polyphosphate Intermediates in the Dephosphorylation of Phytic Acid by Acid Phosphatase with Phytase Activity from Rice Bran
Hayakawa, Toshiro,Suzuki, Kayoko,Miura, Hidehiko,Ohno, Takao,Igaue, Ikuo
, p. 279 - 286 (2007/10/02)
Myo-inositol phosphate intermediates formed during desphosphorylation of phytic acid (by rice bran acid phosphatase with phytase activity F2) were isolated by ion-exchange chromatography.Hydrolysis of phytic acid proceeded via L-myo-inositol 1,2,3,4,5 and D-myo-inositol 1,2,4,5,6-pentakisphosphate, D-myo-inositol 1,2,5,6 and L-myo-inositol 1,2,3,4-tetrakisphosphate, D-myo-inositol 1,2,6 and myo-inositol 1,2,3-trisphosphate, D-myo-inositol 1,2-bisphosphate and finally myo-inositol monophosphate (myo-inositol 2, 93percent; D-myo-inositol 1, 7percent).Six percent of myo-inositol and 85percent of Pi were found in the reaction mixture when the hydrolyses of phytic acid by the enzym did not proceed further.The initial points of dephorylation of phytic acid by the phytase took place at L-myo-inositol 6 (85percent), D-myo-inositol 3 (7percent), D-myo-inositol 2 (5percent), and myo-inositol 5 (3percent) at the positions of the myo-inositol ring.
