87-89-8Relevant academic research and scientific papers
Forced degradation of L-(+)-bornesitol, a bioactive marker of Hancornia speciosa: Development and validation of stability indicating UHPLC-MS method and effect of degraded products on ACE inhibition
Gomes, José Hugo de Sousa,da Silva, Grazielle Caroline,C?rtes, Steyner F.,de Pádua, Rodrigo Maia,Braga, Fern?o Castro
, p. 31 - 38 (2018)
The antihypertensive activity of the medicinal plant Hancornia speciosa has been previously demonstrated by us, being the activity ascribed to polyphenols and cyclitols like L-(+)-bornesitol. We herein evaluated the stability of the bioactive marker bornesitol submitted to forced degradation conditions. Bornesitol employed in the study was isolated from H. speciosa leaves. An UHPLC-ESI-MS/MS method was developed to investigate bornesitol stability based on MRM (Multiple Reaction Monitoring) acquisition mode and negative ionization mode, employing both specific (m/z 193 → 161 Da) and confirmatory (m/z 193 → 175 Da) transitions. A gradient elution of 0.1% formic acid in water and acetonitrile was performed on a HILIC column. The method was validated and showed adequate linearity (r2 > 0.99), selectivity, specificity, accuracy, and precision (RSD 2.9%). The method was robust for deliberate variations on dessolvation temperature, but not for changes in the flow rate and dessolvation gas. The results from the stability studies allowed us to classify bornesitol as labile for acidic and alkaline hydrolysis, but as very stable for oxidative and neutral hydrolysis exposure. Bornesitol was categorized as practically stable under photolysis degradation, whereas a considerable reduction on its contents was induced by metal ions and thermolysis exposure. Degraded samples from neutral hydrolysis and thermolysis were assayed in vitro for ACE inhibition and showed a substantial decrease in biological activity as compared to intact bornesitol. myo-Inositol was identified as the major degradation products in both matrices. This is the first report on bornesitol stability under different stress conditions and the obtained data are relevant for the development and quality control of standardized products from H. speciosa leaves.
Analysis of metabolically labeled inositol phosphate messengers by NMR
Puschmann, Robert,Harmel, Robert K.,Fiedler, Dorothea
, p. 35 - 52 (2020/06/23)
Inositol phosphates (InsPs) are an important group of eukaryotic messengers and mediate a wide range of processes. To elucidate the biological functions of these molecules, robust techniques to characterize inositol phosphate metabolism at the cellular level are highly sought after. This chapter provides a detailed protocol for the preparation of 13C-labeled myo-inositol, its use for metabolic labeling of mammalian and yeast cells, and the quantitative analysis of intracellular InsP pools from cell extracts using NMR spectroscopy.
METHODS OF EXTRACTING PHOSPHORUS FROM DISTILLATES
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Paragraph 0029; 0099, (2016/11/28)
Methods of processing distillates, methods of removing at least some portion of total phosphorus in a distillate, methods of removing at least some portion of the soluble inorganic phosphorus, phytate phosphorus, or some combination thereof in a distillate, methods for obtaining phytate from distillates, methods for producing phytate derivatives and combinations thereof.
Method for the production of inositol phytin
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Paragraph 0021-0024, (2017/03/17)
The invention discloses a method for preparing inositol by adopting phytine. The method comprises the following steps: with the phytine as a raw material, adding deionized water and the phytine into a reactor according to a weight ratio of (5-20):1 of liquid to material, regulating the pH value to 4-5.5, adding phytase of which the weight is 0.5-3 percent of that of the phytine, stirring for hydrolysis under the assistance of ultrasonic waves, and controlling the temperature of a reaction system to be 37-50 DEG C, wherein the action time of the ultrasonic waves is 0.5-1 hour in the hydrolysis process, and the start time of the action of the ultrasonic waves is 0-50 percent of the total reaction time; ending the hydrolysis reaction after the hydrolysis is performed for 4-24 hours, adding solid super-acid of which the weight is 0.2-1 percent of that of the phytine into hydrolysate, performing complete hydrolysis for 15-45 minutes under a pressure of 0.12-0.20 MPa at the reaction temperature of 105-120 DEG C, filtering, adding filtrate into a water solution of calcium oxide, regulating the pH to 7.0, filtering, concentrating the filtrate until the ratio of the liquid to the material is 2:1 based on the initial weight of the phytine, then adding ethanol in a plurality of steps and respectively separating and precipitating to finally obtain the inositol. The method disclosed by the invention has the advantages of low pressure, low temperature and low energy consumption.
Elaboration of the ether cleaving ability and selectivity of the classical Pearlman's catalyst [Pd(OH)2/C]: Concise synthesis of a precursor for a myo-inositol pyrophosphate
Mart, Alson,Shashidhar, Mysore S.
, p. 9769 - 9776,8 (2012/12/11)
The cleavage of propargyl, allyl, benzyl, and PMB ethers by Pd(OH) 2/C can be tuned in that order, by varying the reaction conditions. Other moieties such as C-C double bonds, esters, trityl ether, p-bromo and p-nitrobenzyl ethers are stable to these reaction conditions. Cleavage of allyl ethers can be made catalytic by using 1:1 mixture of Pd(OH)2/C and Pd/C. The synthetic potential of the selective ether cleaving ability of Pd(OH)2/C, essentially under neutral conditions, has been demonstrated by an efficient synthesis of a precursor for the preparation of an inositol pyrophosphate derivative.
Elaboration of the ether cleaving ability and selectivity of the classical Pearlman's catalyst [Pd(OH)2/C]: Concise synthesis of a precursor for a myo-inositol pyrophosphate
Mart, Alson,Shashidhar, Mysore S.
, p. 9769 - 9776 (2013/01/13)
The cleavage of propargyl, allyl, benzyl, and PMB ethers by Pd(OH) 2/C can be tuned in that order, by varying the reaction conditions. Other moieties such as C-C double bonds, esters, trityl ether, p-bromo and p-nitrobenzyl ethers are stable to these reaction conditions. Cleavage of allyl ethers can be made catalytic by using 1:1 mixture of Pd(OH)2/C and Pd/C. The synthetic potential of the selective ether cleaving ability of Pd(OH)2/C, essentially under neutral conditions, has been demonstrated by an efficient synthesis of a precursor for the preparation of an inositol pyrophosphate derivative.
Protecting group directed stereoselective reduction of an epi-inosose: Efficient synthesis of epi-inositol
Patil, Madhuri T.,Krishnaswamy, Shobhana,Sarmah, Manash P.,Shashidhar, Mysore S.
supporting information; experimental part, p. 3756 - 3758 (2011/08/06)
A facile and high yielding synthesis of epi-inositol via stereoselective reduction of a pentaprotected epi-inosose is reported. Extent of stereoselectivity during the hydride reduction appears to depend on the ability of the substrate to complex with metal ions in the reducing agent.
In vitro and in vivo antioxidant activity of a water-soluble polysaccharide from dendrobium denneanum
Luo, Aoxue,Ge, Zhongfu,Fan, Yijun,Luo, Aoshuang,Chun, Ze,Jin He, Xing
experimental part, p. 1579 - 1592 (2011/04/25)
The water-soluble crude polysaccharide (DDP) obtained from the aqueous extracts of the stem of Dendrobium denneanum through hot water extraction followed by ethanol precipitation, was found to have an average molecular weight (Mw) of about 484.7 kDa. Monosaccharide analysis revealed that DDP was composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.66:8.92:34.20:10.16. The investigation of antioxidant activity both in vitro and in vivo showed that DDP is a potential antioxidant.
Conformational study of the natural iron chelator myo-inositol 1,2,3-trisphosphate using restrained/flexible analogues and computational analysis
Mansell, David,Veiga, Nicolás,Torres, Julia,Etchells, Laura L.,Bryce, Richard A.,Kremer, Carlos,Freeman, Sally
experimental part, p. 8949 - 8957 (2011/01/04)
Myo-Inositol 1,2,3-trisphosphate [Ins(1,2,3)P3], a component in mammalian cells, possesses the correct chemical properties of an intracellular iron transit ligand. Here we have examined the conformation of the Ins(1,2,3)P3-Fe3+ complex. The synthesis and antioxidant properties of 4,6-carbonate-myo-inositol 1,2,3,5-tetrakisphosphate [4,6-carbonate Ins(1,2,3,5)P4], which is locked in the unstable penta-axial chair conformation and 1,2,3-trisphosphoglycerol, a flexible acyclic analogue of Ins(1,2,3)P3, are reported. 4,6-Carbonate Ins(1,2,3,5)P4 caused complete inhibition of iron-catalysed hydroxyl radical (HO?) formation at 100 μM, thereby resembling Ins(1,2,3)P3 and supporting a penta-axial chair binding conformation. In contrast, 1,2,3-trisphosphoglycerol was shown to have incomplete antioxidant properties. In support of experimental observations, we have applied high-level density functional calculations to the binding of Ins(1,2,3)P3 to iron. This study provides evidence that Fe3+ binds tightly to the less stable penta-axial conformation of Ins(1,2,3)P3 using terminal and bridging phosphate oxygens, thought to also contain a tightly bound water molecule or hydroxyl ligand in the complex.
BIOOCOMPATIBLE POLYMER COMPOSITIONS
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, (2010/01/31)
The present invention provides a biocompatible prepolymer comprising hydrophilic and hydrophobic segments, wherein the hydrophobic segments have at least one ethylenically unsaturated functional group and at least 5% of the segments have two or more ethylenically unsaturated functional groups and water. The invention further provides a biocompatible prepolymer composition comprising hydrophilic and hydrophobic prepolymers, wherein at least one of the hydrophobic prepolymers has at least one ethylenically unsaturated functional group and at least 5% of the prepolymers have two or more ethylenically unsaturated functional groups and water. The invention further provides use of the prepolymer or prepolymer compositions of the invention in biomedical applications such as tissue engineering, as bone substitutes or scaffolds, and in wound treatment.
