94367-21-2 Usage
Uses
Used in Pharmaceutical Industry:
SUC-LEU-LEU-VAL-TYR-AMC is used as a diagnostic tool for monitoring the activity of chymotrypsin-like proteases and calpains, which are involved in various cellular processes and can be associated with diseases. By assessing the activity of these enzymes, researchers and clinicians can gain insights into the underlying mechanisms of certain conditions and evaluate the effectiveness of potential therapeutic interventions.
Used in Research Applications:
In the field of research, SUC-LEU-LEU-VAL-TYR-AMC serves as a valuable substrate for studying the function and regulation of proteasomes and calpains. It can be employed in various experimental setups, such as enzyme assays, to investigate the role of these proteases in cellular processes and their potential as therapeutic targets.
Used in Tuberculosis Treatment:
SUC-LEU-LEU-VAL-TYR-AMC is used as a therapeutic agent for treating tuberculosis. Its ability to target and modulate the activity of specific proteases involved in the disease's progression makes it a promising candidate for developing new treatment strategies against this infectious disease.
Biochem/physiol Actions
In the presence of chymotrypsin-like enzyme activity, the fluorophore, 7-amido-4-methylcoumarin is released from N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin. The fluorescence obtained is a measure of the enzyme activity.6
References
1) Stein et al. (1996), Kinetic characterization of the chymotryptic activity of the 20S proteasome; Biochemistry, 35 3899
2) Sasaki et al. (1984), Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates; J. Biol. Chem., 259 12489
3) Hamouda et al. (2014), The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells; Oncotarget, 5 6252
4) Min et al. (2017), USP14 inhibitor attenuates cerebral ischemia/reperfusion-induced neuronal injury in mice; J. Neurochem,, 140 826
Check Digit Verification of cas no
The CAS Registry Mumber 94367-21-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,4,3,6 and 7 respectively; the second part has 2 digits, 2 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 94367-21:
(7*9)+(6*4)+(5*3)+(4*6)+(3*7)+(2*2)+(1*1)=152
152 % 10 = 2
So 94367-21-2 is a valid CAS Registry Number.
InChI:InChI=1/C40H53N5O10/c1-21(2)16-29(42-33(47)14-15-34(48)49)38(52)43-30(17-22(3)4)39(53)45-36(23(5)6)40(54)44-31(19-25-8-11-27(46)12-9-25)37(51)41-26-10-13-28-24(7)18-35(50)55-32(28)20-26/h8-13,18,20-23,29-31,36,46H,14-17,19H2,1-7H3,(H,41,51)(H,42,47)(H,43,52)(H,44,54)(H,45,53)(H,48,49)/t29?,30?,31-,36-/m0/s1
