945633-30-7

Usage

Uses

Used in Bioconjugation Applications:
Biotin-PEG3-N3 is used as a bioconjugation agent for the specific attachment of biomolecules, such as proteins, peptides, or nucleic acids, to avidin or streptavidin. The biotin-avidin/streptavidin interaction ensures high affinity and stability, making it suitable for various applications, including diagnostics, therapeutics, and research.
Used in Drug Delivery Systems:
In the pharmaceutical industry, Biotin-PEG3-N3 is used as a targeting moiety for the development of drug delivery systems. The biotin molecule can be used to specifically target cells or tissues expressing high levels of avidin or streptavidin, allowing for targeted drug delivery and reducing side effects.
Used in Biosensor Development:
Biotin-PEG3-N3 is used as a key component in the development of biosensors. The biotin-avidin/streptavidin interaction provides a stable and specific binding platform, while the PEG chain enhances the solubility and stability of the biosensor. The azide group allows for the attachment of various functional groups or reporter molecules, enabling the detection and quantification of target analytes.
Used in Cell Biology Research:
In cell biology research, Biotin-PEG3-N3 is used as a labeling agent for the visualization and tracking of cellular components. The biotin molecule can be attached to specific cellular targets, while the PEG chain and azide group provide additional functionality, such as the attachment of fluorescent dyes or other reporter molecules for imaging studies.

Upstream product

• 166388-57-4 2-[2-(2-azidoethoxy)ethoxy]ethanamine 
• 59559-06-7 1,8-diazido-3,6-dioxaoctane 
• 58-85-5 biotin 
• 929537-66-6 C₂HF₃O₂*C₆H₁₄N₄O₂ 

Relevant academic research and scientific papers

The Synthesis and Biological Evaluation of Some C-9 and C-10 Substituted Derivatives of the RNA Polymerase i Transcription Inhibitor CX-5461

The regio-isomeric alkynyl-substituted derivatives, 2 and 3, of the RNA Polymerase I (Pol I) transcription inhibitor CX-5461 (1) were prepared and the active one (compound 3) subjected to click reactions ([3 + 2]-cycloaddition reactions) with certain alkyl azides bearing biotin or fluorescent tags. Compounds 2 and 3, as well as four [3 + 2]-cycloadducts of the latter, were subjected to biological evaluation in a human acute myeloid leukemia cell line model. Among the six compounds tested only alkyne 3 remained active but this was less potent than parent 1.

Bilateral biotin-phthalocyanine zinc conjugate as well as preparation and application thereof

The invention discloses a bilateral biotin-phthalocyanine zinc conjugate as well as preparation and application thereof. According to the conjugate disclosed by the invention, a biotin-cell growth-promoting factor (which is a critical micronutrient for cells to maintain normal functions, growth and reproduction) is used as a tumor target and is covalently connected onto a phthalocyanine zinc photosensitizer capable of being used for a photodynamic therapy, thus obtaining a third-generation anti-cancer photosensitizer for targeted therapy. Meanwhile, according to the conjugate, the biotin instead of a phthalocyanine zinc derivative acts a research object, human breast cancer cell MCF-7 and human embryo lung inoblast HELF act as subject cell lines respectively, a research regarding the in-vitro anti-cancer activity of the biotin is developed, and a prodrug suitable for a molecular targeted therapy is screened out, and a foundation is laid for applying the biotin instead of the phthalocyanine zinc derivative to the targeted therapy of cancers. The compound, namely the conjugate disclosed by the invention, is relatively simple in synthesis method, easily available in raw materials, low in cost, fewer in side reactions, relatively high in yield, easy to purify and beneficial for industrial production.

Rational design of biotin-disulfide-coumarin conjugates: A cancer targeted thiol probe and bioimaging

Biotin-disulfide-coumarin conjugates are designed and synthesized as novel fluorescent sensors for cancer targeted intracellular thiol imaging in living organisms. In vitro experiments disclose that probe 6 is preferentially taken up by biotin receptor-positive A549 tumor cells through receptor mediated endocytosis. The Royal Society of Chemistry.

A fluorescence off-on reporter for real time monitoring of gemcitabine delivery to the cancer cells

We present the design, synthesis, optical properties and in vitro biological assessments of the theranostic prodrug 6 in which a near IR fluorophore is conjugated with a cancer cell-directing biotin unit; further it is linked with the anti-cancer drug gemcitabine via a self-immolative spacer, a disulfide bond. The prodrug 6 is able to monitor drug delivery and cellular imaging.

Design, synthesis, and biological evaluation of biotin-labeled (-)-ternatin, a potent fat-accumulation inhibitor against 3T3-L1 adipocytes

The design, synthesis, and biological activity of biotin-labeled (-)-ternatin are reported. Chemical modification, that is, biotinylation, was conducted using Click chemistry at the 6-position (NMe-d-ProGly moiety), which was a plausible location selected