946583-63-7Relevant academic research and scientific papers
Microscale purification in support of high-throughput medicinal chemistry
Barhate, Chandan L.,Black, Regina,Cavallaro, Cullen L.,Davies, Merrill,Donnell, Andrew F.,Li, Ling,Priestley, E. Scott,Weller, Harold N.,Yang, Fukang,Zhang, Yingru,Zhang, Yong,Zipp, Greg
supporting information, p. 11037 - 11040 (2021/10/30)
In recent years, successful assay miniaturization has enabled the exploration of synthesis scale reduction in pharmaceutical discovery. Miniaturization of pharmaceutical synthesis and purification allows a reduction in material consumption and shortens timelines, which ultimately reduces the cost per experiment without compromising data quality. Isolating and purifying the compounds of interest is a key step in the library synthesis process. In this manuscript we describe a high-throughput purification workflow in support of microscale (1-5 μmol or 0.5-2 mg) library synthesis. The optimized microscale purification system can routinely purify 384-well reaction plates with an analysis time of 4 min per sample. Instrument optimization, critical parameters such as column loading, delay time calibration, ultrafast pre- and post-purification analysis and library purification examples are provided.
