948891-63-2Relevant academic research and scientific papers
CDC25A-inhibitory RE derivatives bind to pocket adjacent to the catalytic site
Tsuchiya, Ayako,Asanuma, Miwako,Hirai, Go,Oonuma, Kana,Muddassar, Muhammad,Nishizawa, Eri,Koyama, Yusuke,Otani, Yuko,Zhang, Kam Y. J.,Sodeoka, Mikiko
, p. 1026 - 1034 (2013)
RE derivatives, which are cell-permeable and non-electrophilic dual-specificity protein phosphatase inhibitors developed in our laboratory, inhibit CDC25A/B non-competitively, as determined by means of kinetic experiments. To identify the binding site of RE derivatives, we designed and synthesized the new probe molecule RE142, having a Michael acceptor functionality for covalent bond formation with the enzyme, a biotin tag to enable enrichment of probe-bound peptide(s), and a chemically cleavable linker to facilitate release of probe-bound peptides from avidin beads. LC-MS analysis indicated that RE142 binds to one of the residues Cys384-Tyr386 of CDC25A, within a pocket adjacent to the catalytic site.
