95047-71-5Relevant academic research and scientific papers
Synthesis of New Cationic Schiff Base Complexes of Copper(II) and Their Selective Binding with DNA
Tanaka, Tomohide,Tsurutani, Keiko,Komatsu, Atsushi,Ito, Toyofumi,Iida, Kazumi,Fujii, Yuki,Nakano, Yoshiharu,Usui, Yoshiharu,Fukuda, Yutaka,Chikira, Makoto
, p. 615 - 629 (2007/10/03)
A series of cationic salen-type Schiff base complexes of copper(II), the general formula of which is [Cu{R3N+-CH2-C6H3(O -)-CH=N-}2X]Br2 (R: CH3, C2H5, C3H7, C4H9; X: C2H4, C3H6, C6H10, C6H4, C10H6), was newly prepared, and the molecular structure of complex 1 (R=CH3, X=C2H4) was determined by an X-ray crystal structure analysis. The binding mode and binding constants (Kb) of all the complexes with calf thymus DNA were investigated at an ionic strength of I = 0.05 (NaCl + HEPES buffer, pH = 7.2) using induced CD and the UV-vis spectra. Those complexes with aliphatic X group selectively bound to the groove of DNA, and the Kb's were in the range of 102 - 103 mol dm-3 (site size base pairs n = 7.2 - 7.4). However, those with aromatic X group selectivity intercalated to the base pairs, and the Kb's were in the range of 104 - 105 mol dm-3 (n = 2.8 - 4.0). These binding modes were confirmed based on the salt dependence of Kb's. In addition, 1 and 10 (R = CH3, X = C6H4) were found to exhibit AT-sequence affinity from the induced CD spectra and Kb's for poly(dA-dT)-poly(dA-dT), poly(dG-dC)-poly(dG-dC), and poly(dA-dC)-poly(dT-dG).
