957545-15-2Relevant academic research and scientific papers
Synthesis of branched oligonucleotides with three different sequences using an oxidatively removable tritylthio group
Utagawa, Eri,Ohkubo, Akihiro,Sekine, Mitsuo,Seio, Kohji
, p. 8259 - 8266 (2008/09/17)
(Chemical Equation Presented) We synthesized a three-way branched oligodeoxynucleotide (ODN) 30-mer using a new branch unit with acid-labile DMTr and oxidatively cleavable TrS groups as orthogonal protecting groups. The branched ODN was successfully synthesized using 5-[3,5-bis(trifluoromethyl) phenyl]-l H-tetrazole and (2R,8aS)-(+)-(camphorylsulfonyl)oxaziridine as the activator of phosphoramidite units and the oxidizing reagent, respectively. We also found that the TrS group was orthogonal to the Lev, TBDMS, and Fmoc groups. These results indicate the possibility of the synthesis of more complex four- and five-way branched ODNs by the combined use of DMTr, TrS, Lev, TBDMS, and Fmoc groups.
