96013-00-2 Usage
Uses
Used in Biochemistry Research:
Uridine 5'-triphosphate, ammonium salt is used as a research tool for studying the mechanisms of RNA and DNA synthesis, as well as the regulation of cellular processes. Its involvement in gene expression and energy transfer makes it a valuable compound for understanding the fundamental aspects of cellular biology.
Used in Pharmaceutical Development:
URIDINE 5'-TRIPHOSPHATE, AMMONIUM SALT is used as a precursor in the synthesis of various nucleotides and nucleic acid analogs, which are essential for the development of new drugs targeting specific genetic disorders and diseases. Its role in neurotransmitter regulation also makes it a potential candidate for the development of therapies for neurological conditions.
Used in Molecular Biology:
Uridine 5'-triphosphate, ammonium salt is used as a component in various molecular biology techniques, such as polymerase chain reaction (PCR) and reverse transcription (RT), where it serves as a substrate for the synthesis of DNA and RNA strands.
Used in Diagnostic Applications:
URIDINE 5'-TRIPHOSPHATE, AMMONIUM SALT can be used in diagnostic assays to detect and measure the activity of enzymes involved in nucleic acid metabolism, providing valuable information about the health and functioning of cells and tissues.
Used in Gene Therapy:
Uridine 5'-triphosphate, ammonium salt may be employed in gene therapy approaches, where it can be used to facilitate the delivery and expression of therapeutic genes, potentially leading to the treatment of genetic disorders and other diseases.
Check Digit Verification of cas no
The CAS Registry Mumber 96013-00-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,6,0,1 and 3 respectively; the second part has 2 digits, 0 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 96013-00:
(7*9)+(6*6)+(5*0)+(4*1)+(3*3)+(2*0)+(1*0)=112
112 % 10 = 2
So 96013-00-2 is a valid CAS Registry Number.
96013-00-2Relevant academic research and scientific papers
Synthesis of Nucleoside Triphosphates from 2′-3′-Protected Nucleosides Using Trimetaphosphate
Mohamady, Samy,Taylor, Scott D.
supporting information, p. 580 - 583 (2016/02/18)
Chemists have been attempting to triphosphorylate nucleosides and other alcohols using trimetaphosphate (TriMP) since the 1960s. However, this route appears to have been abandoned due to poor yields. The first practical syntheses of nucleoside triphosphates (NTPs) are reported using TriMP as the key reagent. This was achieved by reacting the tetrabutylammonium salt of TriMP with mesitylenesulfonyl chloride in the presence of DABCO in pyridine followed by the addition of an appropriately protected nucleoside and phthalimide. Quenching the reaction with aqueous buffer followed by hydrolysis of the OH protecting groups gave the NTPs in good yield.
A P(V)-N activation strategy for the synthesis of nucleoside polyphosphates
Sun, Qi,Gong, Shanshan,Sun, Jian,Liu, Si,Xiao, Qiang,Pu, Shouzhi
, p. 8417 - 8426 (2013/09/24)
A general and high-yielding synthesis of nucleoside 5′-triphosphates (NTPs) and nucleoside 5′-diphosphates (NDPs) from protected nucleoside 5′-phosphoropiperidates promoted by 4,5-dicyanoimidazole (DCI) has been developed. 31P NMR tracing experiments showed that the sequential deprotection and coupling reactions were exceptionally clean. The phosphoropiperidate exhibited superior reactivity to the conventional phosphoromorpholidate toward DCI-promoted NTP/NDP synthesis. The experimental results suggested that the mechanism of DCI activation could be distinctive for NTP and NDP synthesis, depending on the different nucleophilicity of pyrophosphate and phosphate.
