97004-78-9

Usage

Uses

Used in Biochemistry Research:
3-(2-Benzoxazolyl)umbelliferyl acetate is used as a substrate in enzymatic assays for the detection and measurement of glycosidase enzyme activity. Its fluorogenic properties enable researchers to monitor the activity of these enzymes through the emitted fluorescence, providing insights into enzyme kinetics and specificity.
Used in Pharmaceutical Research:
In the pharmaceutical industry, 3-(2-Benzoxazolyl)umbelliferyl acetate is utilized for the study of enzyme kinetics and inhibitor screening. Its ability to fluoresce upon enzymatic hydrolysis aids in the identification and development of potential enzyme inhibitors, which can be crucial in the discovery of new drugs targeting specific glycosidase enzymes.

Upstream product

• 64887-40-7 3-(benzo[d]oxazol-2-yl)-7-hydroxyl -2H-chromen-2-one 
• 108-24-7 acetic anhydride 
• 75-36-5 acetyl chloride 
• 15344-56-6 2-(benzo[d]oxazol-2-yl)acetonitrile 
• 95-01-2 2,4-Dihydroxybenzaldehyde 

Relevant academic research and scientific papers

Synthesis of Some Coumarin Derivatives as Potential Laser Dyes

With a view to extend the tunability range and maximum output of the coumarin series of dyes, fifteen new 3-substituted 7-hydroxycoumarins emitting in the blue-green region of the visible spectrum are synthesized, and some of them showed laser activity.

Syntheses and Spectral Properties of Longwave Absorbing and Fluorescing Substrates for the Direct and Continuous Kinetic Assay of Carboxylesterases, Phosphatases, and Sulfatases

Syntheses, absorption and fluorescence properties for a series of new enzyme substrates are described, which are derived from 7-hydroxycoumarins possessing electron-withdrawing substituents in position 3.The new substrates are advantageous over existing ones in that they exhibit longwave absorption and fluorescence maxima as well as large Stokes' shifts.In addition, their pKa values, which are usually between 6.0 and 7.0, allow the direct and continuous kinetic assay of hydrolases such as esterases, phosphatases, and sulfatases. - Keywords: Enzyme substrates; Absorption spectra; Fluorescence spectra