97739-37-2Relevant academic research and scientific papers
Isotope-Coded Maleimide Affinity Tags for Proteomics Applications
Wdowiak, Adam P.,Duong, Marisa N.,Joyce, Rohan D.,Boyatzis, Amber E.,Walkey, Mark C.,Nealon, Gareth L.,Arthur, Peter G.,Piggott, Matthew J.
, p. 1652 - 1666 (2021/07/20)
Isotope-coded affinity tags (ICATs) are valuable tools for mass spectrometry-based quantitative proteomics, in particular, for comparison of protein (cysteine-residue) thiol oxidation state in normal, stressed, and diseased tissue. However, the iodoacetam
Pd(II)-Mediated C?H Activation for Cysteine Bioconjugation
Frost, Christopher G.,Kociok-K?hn, Gabriele,Lubben, Anneke T.,Reeksting, Shaun B.,Tilden, James A. R.
supporting information, (2022/01/19)
Selective bioconjugation remains a significant challenge for the synthetic chemist due to the stringent reaction conditions required by biomolecules coupled with their high degree of functionality. The current trailblazer of transition-metal mediated bioconjugation chemistry involves the use of Pd(II) complexes prepared via an oxidative addition process. Herein, the preparation of Pd(II) complexes for cysteine bioconjugation via a facile C?H activation process is reported. These complexes show bioconjugation efficiency competitive with what is seen in the current literature, with a user-friendly synthesis, common Pd(II) sources, and a more cost-effective ligand. Furthermore, these complexes need not be isolated, and still achieve high conversion efficiency and selectivity of a model peptide. These complexes also demonstrate the ability to selectively arylate a single surface cysteine residue on a model protein substrate, further demonstrating their utility.
