99692-48-5

Upstream product

• 2338-12-7 5-nitrobenzotriazole 
• 50408-20-3 2',3'-di-O-benzoyluridine 
• 65109-15-1 2'-O-t-Butyldimethylsilyl-5'-O-monomethoxytrityl-N⁶-benzoyluridin 
• 15074-54-1 2-chlorophenyl dichlorophosphate 

Downstream Products

• 112343-27-8 C₄₄H₄₈ClN₄O₁₆PSi 

Relevant academic research and scientific papers

Formation of Interribonucleoside Phosphate Bond by the Use of Reagent Formed by the Reaction of 2-Chlorophenyl Phosphorodichloridate with 5-Nitrobenzotriazole and Preparation of Anticodon Triplet of Yeast tRNALys

The reagent formed by the reaction of 2-chlorophenyl phosphorodichloridate with an equimolar amount of 5-nitrobenzotriazole was treated with protected ribonucleosides having free 3'-OH, followed by protected ribonucleosides having free 5'-OH in pyridine for 8 h (the second step), the corresponding dinucleoside phosphates being obtained in 55-84 percent yields.When the second step was carried out in the presence of 2,6-lutidine, the yields were increased to 80-90 percent within 2 h.By this procedure, the anticodon triplet of yeast tRNALys was prepared in 71 percent yield.

A CONVENIENT PROCEDURE FOR THE PREPARATION OF DINUCLEOSIDE PHOSPHATES BY THE USE OF A BIFUNCTIONAL PHOSPHORYLATING REAGENTS, 2-CHLOROPHENYLPHOSPHOROBIS(NITROBENZOTRIAZOLIDE)

The reagent formed by the reaction of 2-chlorophenyl phosphorodichloridate with 2 molar amounts of 5-nitrobenzotriazole was allowed to react successively with nucleosides having free 3'-hydroxyl groups and nucleosides having free 5'-hydroxyl groups affording the corresponding dinucleoside phosphates in 63-97 percent yields.