Reference

Specificity of dye-ligand interaction with the polynucleotide-binding domain of 1,25-dihydroxyvitamin D3-receptor complexes of chicken intestinal cytosol

Specificity of dye-ligand interaction with the polynucleotide-binding domain of 1,25-dihydroxyvitamin D3-receptor complexes of chicken intestinal cytosol. Mellon, William S. (Sch. Pharm., Univ. Wisconsin, Madison, WI 53706, USA). Mol. Pharmacol., 25(1), 86-91 (English) 1984. CODEN: MOPMA3. ISSN: 0026-895X.In this article, certain chemicals are used. Some of their cas registry numbers are 9004-34-6 and 24991-23-9 DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) The binding of 1,25-dihydroxyvitamin D3-receptor complexes from chick intestinal cytosol to DNA-cellulose and isolated intestinal nuclei is inhibited by several dye-ligands in a dose-dependent manner. Concns. of Cibacron blue F3GA [12236-82-7], blue dextran [9049-32-5], Procion red HE3B [61951-82-4], and Green A [12000-19-0] dye causing 50% competition for receptor binding to DNA-cellulose ranged 2.8-3.6 mM. A structural analog of the anthraquinone moiety of Cibacron blue F3GA, bromaminic acid [116-81-4], was 111-fold less potent in inhibiting DNA-cellulose binding. Moreover, the inhibitory effects of these dye-ligands is not due to a simple electrostatic effect, since 2 other polyanions, heparin [9005-49-6] and poly-L-glutamate [25513-46-6], are much less effective. Whereas dye-ligands can cause the release of receptors bound to DNA-cellulose, they do not alter the dissocn. of 1,25-dihydroxyvitamin D3 from its receptor nor do they affect the apparent equil. binding const. of the receptor or the concn. of available sterol-binding sites. The inhibition of binding by dye-ligands is competitive with respect to DNA-cellulose binding, indicating that the effect of these dyes is at a domain common to polynucleotides. .

1,4-Diaminoanthraquinone-2-sulfonic acid or its salts

1,4-Diaminoanthraquinone-2-sulfonic acid or its salts. Hattori, Makoto; Taguma, Akihiro; Takeshita, Akira (Sumitomo Chemical Co., Ltd. , Japan). Ger. Offen. DE 3328046 A1 9 Feb 1984, 10 pp. (German). (Germany). CODEN: GWXXBX. CLASS: IC: C07C143-665. APPLICATION: DE 83-3328046 3 Aug 1983. PRIORITY: JP 82-136484 4 Aug 1982. DOCUMENT TYPE: Patent CA Section: 41 (Dyes, Organic Pigments, Fluorescent Brighteners, and Photographic Sensitizers) The presence of an inert solvent, e.g. n-heptane [142-82-5] or cyclohexane [110-82-7], in the reaction of 1-amino-4-bromoanthraquinone-2-sulfonic acid (I) [116-81-4] (or its salts) with liq. NH3 in the presence of a Cu catalyst gives 1,4-diaminoanthraquinone-2-sulfonic acid (II) [4095-85-6] in high yield and purity and without crystal deposition on the reactor walls. For example, a mixt. of n-heptane 220, I Na salt [6258-06-6] (94% purity) 163.6, CuO 3, and liq. NH3 300 parts was heated at 70° for 8 h to give II (98. 78-78-4 and 6258-06-6 are just another two chemicals used in this study.3% purity) in 99.0% yield. .