Detail of "1464-53-5"

Reference

Assay of cytotoxicity and mutagenicity of alkylating agents by using Neurospora spheroplasts

Assay of cytotoxicity and mutagenicity of alkylating agents by using Neurospora spheroplasts. Pope, Susan; Baker, Jennifer M.; Parish, J. H. (Dep. Biochem., Univ. Leeds, Leeds LS2 9JT, UK). Mutat. Res., 125(1), 43-53 (English) 1984. CODEN: MUREAV. ISSN: 0027-5107. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Mutagenicity was assayed by using reversion of alleles in the am gene selected to recognize certain specified transitions and also undefined point mutations. Cytotoxicity was quantified by measuring a cytotoxic parameter, m, which appears in the exponential function that fits the survival/dose curve for each compd. (under std. incubation conditions). Of the compds. tested, nitrogen mustard [55-86-7] was cytotoxic and nonmutagenic; and ENU [759-73-9] was highly mutagenic and noncytotoxic. Of the remaining compds., methylnitrosourea [684-93-5], butadiene diepoxide [1464-53-5], and cis-platin [15663-27-1], all showed comparable mutagenicity/survivor, although the m values covered a wide range. Differences were found between the different compds. in the effects of the uvs-2 allele on survival and the preponderance of G to A transitions.

Comparison of rat and hamster hepatocyte primary culture/DNA repair assays

Comparison of rat and hamster hepatocyte primary culture/DNA repair assays. Kornbrust, Douglas J.; Barfknecht, Thomas R. (Res. Triangle Inst., Research Triangle Park, NC 27709-2194, USA). Environ. Mutagen., 6(1), 1-11 (English) 1984. CODEN: ENMUDM. ISSN: 0192-2521. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) A comparison of the relative extent of DNA repair elicited by various genotoxic chems. 5522-43-0 and 134-32-7 which are cas registry numbers are also used here. in rat and hamster hepatocytes was conducted, using the hepatocyte primary culture/DNA repair (HPC/DR) assay. Of the 11 chems. tested, 8 were more potent in inducing DNA repair in hamster hepatocytes than in rat hepatocytes. Dimethylnitrosamine [62-75-9], diethylnitrosamine [55-18-5], 2-acetylaminofluorene [53-96-3], 9-aminoacridine [90-45-9], pararosaniline [569-61-9], 1-naphthylamine [134-32-7], benzidine [92-87-5], and 1,2:3,4-diepoxybutane [1464-53-5] were all active in hamster hepatocytes at a concn. at least 10 times less than the lowest effective concn. in rat hepatocytes. The direct-acting alkylating agent, Me methanesulfonate [66-27-3], was equipotent in inducing DNA repair in both rat and hamster hepatocytes, indicating that the differences in DNA repair obsd. for the other chems. were probably not a result of species differences in DNA repair capacities. In contrast, 1-nitropyrene [5522-43-0] produced a greater DNA repair response in rat hepatocytes than hamster hepatocytes, whereas the bacterial mutagen 3-(chloromethyl)pyridine [3099-31-8] was inactive in both hepatocyte systems. These studies demonstrate the feasibility of using hamster hepatocytes in the HPC/DR assay and illustrate the utility of performing the assay with hepatocytes from >1 species. .