Reference

Photocolorimetric method for determination of chlorhexidine gluconate in antiseptic preparations

Photocolorimetric method for determination of chlorhexidine gluconate in antiseptic preparations. Sukiasyan, A. N.; Kopylova, A. I.; Malysheva, L. F. (VNII Dez. Steril., Moscow, USSR). Khim.-Farm. Zh., 18(10), 1271-3 (Russian) 1984. CODEN: KHFZAN. ISSN: 0023-1134. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Photometric detn. of chlorhexidine gluconate [18472-51-0] involved addn. of a K phthalate buffer (pH 3.8-4.2), water, and sulfonitrazo E or sulfonitrazo R to an aliquot of the drug soln. in water and measurement of the absorbance. The drug-dye complexes have the highest absorbances at 600 nm and 610 nm, resp.; the Beer-Lambert law was obsd. at the drug concn. of 200-600 mg/mL. At concns. <200 mg/mL, the anal. error was high and at concns. >600 mg/mL, the drug pptd. from the soln. The anal. error was <1.4%. Chlorhexidine gluconate could also analyzed in antiseptic formulations.

Experimental evaluation of chlorhexidine gluconate for ocular antisepsis

Experimental evaluation of chlorhexidine gluconate for ocular antisepsis. Hamill, M. Bowes; Osato, Michael S.; Wilhelmus, Kirk R. (Cullen Eye Inst., Baylor Coll. Med., Houston, TX 77030, USA). Antimicrob. Agents Chemother., 26(6), 793-6 (English) 1984. CODEN: AMACCQ. ISSN: 0066-4804. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Section cross-reference(s): 10 Chlorhexidine gluconate [18472-51-0] was evaluated for retardation of epithelial regeneration after exptl. corneal abrasion in rabbits. Irrigant concns. of 2.0 and 4.0% chlorhexidine significantly slowed the healing rate (0.546 and 0.076 mm/h, resp.) as compared with saline controls (0.938 mm/h). Irrigant concns. of ￡1% did not delay healing. In a sep. group of animals, the right conjunctivae of pigmented rabbits were inoculated with Staphylococcus epidermidis (~107 organisms/eye) and irrigated with 40 mL of aq. chlorhexidine in concns. of 0.1, 0.5, and 1.0%; the left eyes were irrigated with saline or left untreated. Quant. conjunctival cultures were obtained, and the total no. of organisms recovered/eye was calcd. All chlorhexidine-treated eyes showed significant redn. in organisms compared with either untreated or saline-irrigated control eyes. In vitro antimicrobial susceptibility testing demonstrated chlorhexidine in concns. of 0.1-4% to be highly active against a variety of gram-pos. and gram-neg. bacterial pathogens. Topical aq. chlorhexidine may be an alternate agent for preoperative conjunctival antisepsis.