Reference

Establishment of a multidrug-resistant cell line BEL-7402/5-FU of human hepatocellular carcinoma and its biological characteristics

All Rights Reserved. Establishment of a multidrug-resistant cell line BEL-7402/5-FU of human hepatocellular carcinoma and its biological characteristics. Gu, Wei; Zhang, Ya-Ni; Li, Bai; Han, Jie; Cheng, Bin-Bin; Ling, Chang-Quan (Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, Peop. Rep. China). Zhongxiyi Jiehe Xuebao, 4(3), 265-270 (Chinese) 2006 Zhongxiyi Jiehe Xuebao Zazhishe. CODEN: ZJXHAY. ISSN: 1672-1977. DOCUMENT TYPE: Journal CA Section: 14 (Mammalian Pathological Biochemistry) Establishment of multidrug-resistant cell line BEL-7402/5-FU of hepatocellular carcinoma (HCC) is studied. BEL-7402/5-FU was induced by pulse therapy combined with continuous stepwise exposure to 5-fluorouracil in vitro. MTT assay was used to det. its multidrug resistance (MDR). Biol. characteristics of the BEL-7402/5-FU cell line were obsd. including morphol. changes, cell growth curve, population doubling time, plate cloning efficiency, adherence rate, cell cycle distribution, chromosome and tumorigenicity. Accumulation amt. of adriamycin (ADM) in cytoplasm was measured by flow cytometry. The protein expression of thymidylate synthase (TS) was evaluated by immuno-cytochem. method. The acquired MDR cell line of BEL-7402/5-FU was established successfully. 19685-09-7 and 9031-61-2 which are cas registry numbers of chemicals are mentioned. The BEL-7402/5-FU cells showed cross-resistance to ADM, vincristine (VCR), methotrexate (MTX) and oxaliplatin (OHP), whereas still sensitive to hydroxycamptothecin (HCPT). The BEL-7402/5-FU cells tended to grow in clusters in vitro. It was found that the population doubling time of BEL-7402/5-FU cells was longer than that of its parental cells. The plate cloning efficiency and the adherence rate of BEL-7402/5-FU cells at the 2nd and 3rd hour were both lower than those of the parental cells. The distributing proportion of BEL-7402/5-FU cells in G0/G1 phase was less than that of the parental cells, whereas the distributing proportion of BEL-7402/5-FU cells in S phase was higher than that of the parental cells. The accumulation amt. of ADM in cytoplasm of BEL-7402/5-FU cells was significantly lower while the expression level of TS protein of which was highly up-regulated as compared with those of the parental cells. Establishment of the human HCC cell line BEL-7402/5-FU might be beneficial to the studies of 5-Fluorouracil acquired MDR mechanisms and the selection of reversal modifiers. .

The thermodynamic dissociation constants of the anticancer drugs camptothecine, 7-ethyl-10-hydroxycamptothecine, 10-hydroxycamptothecine and 7-ethylcamptothecine by the least-squares nonlinear regression of multiwavelength spectrophotometric pH-titration data

All Rights Reserved. The thermodynamic dissociation constants of the anticancer drugs camptothecine, 7-ethyl-10-hydroxycamptothecine, 10-hydroxycamptothecine and 7-ethylcamptothecine by the least-squares nonlinear regression of multiwavelength spectrophotometric pH-titration data. Meloun, Milan; Bordovska, Sylva; Vrana, Ales (Department of Analytical Chemistry, University of Pardubice, Pardubice CZ 532 10, Czech Rep.). Analytica Chimica Acta, 584(2), 419-432 (English) 2007 Elsevier B.V. CODEN: ACACAM. ISSN: 0003-2670. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Section cross-reference(s): 1 The mixed dissocn. consts. of four anticancer drugs - camptothecine, 7-ethyl-10-hydroxycamptothecine, 10-hydroxycamptothecine and 7-ethylcamptothecine, including diprotic and triprotic mols. at various ionic strengths I of range 0.01 and 0.4, and at temps. of 25 and 37° - were detd. with the use of two different multiwavelength and multivariate treatments of spectral data, SPECFIT32 and SQUAD(84) nonlinear regression analyses and Indexes factor anal. A proposed strategy for dissocn. consts. detn. is presented on the acid-base equil. of camptothecine. Indexes of precise modifications of the factor anal. in the program Indexes predict the correct no. of components, and even the presence of minor ones, when the data quality is high and the instrumental error is known. The thermodn. dissocn. const. pKTa was estd. by nonlinear regression of {pKa, I} data at 25 and 37°: for camptothecine pKTa,1 = 2.90(7) and 3.02(8), pKTa,2 = 10.18(30) and 10.23(8); for 7-ethyl-10-hydroxycamptothecine, pKTa,1 = 3.11(2) and 2.46(6), pKTa,2 = 8.91(4) and 8.74(3), pKTa,3 = 9.70(3) and 9.47(8); for 10-hydroxycamptothecine pKTa,1 = 2. 19685-09-7 and 86639-52-3 which are cas registry numbers of chemicals are mentioned.93(4) and 2.84(5), pTa,2 = 8.93(2) and 8.92(2), pKTa,3 = 9.45(10) and 9.98(4); and for 7-ethylcamptothecine pKTa,1 = 3.10(4) and 3.30(16), pKTa,2 = 9.94(9) and 10.98(18). Goodness-of-fit tests for various regression diagnostics enabled the reliability of the parameter ests. found to be proven. Pallas and Marvin predict pKa being based on the structural formulas of drug compds. in agreement with the exptl. value. .