Reference

Quality control studies of berberine hydrochloride by reversed-phase HPLC

Quality control studies of berberine hydrochloride by reversed-phase HPLC. Zhou, Yuanyao; Li, Fang (Wuhan Munic. Inst. Drug Control, Wuhan, Peop. Rep. China). Yaowu Fenxi Zazhi, 4(3), 131-4 (Chinese) 1984. CODEN: YFZADL. ISSN: 0254-1793. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Berberine (I) [2086-83-1], palmatine [3486-67-7], jatrorrhizine [3621-38-3] and berberubine [17388-19-1] were sepd. on reversed-phase high-performance liq. chromatog. equipped with a LC-65T detector. Nucleosil 10 C18 (plate no. ~3000) was used as stationary phase and 0.02 M H3PO4 contg. 28% acetonitrile as mobile phase. Good resoln. was achieved. Of 41 batches of I-HCl prepns. tested, all contained jatrorrhizine (2.3-10.6%) and berberubine (0.3-1.2%). Trace amts. of palmitine (<1.1%) also were found in some samples. The color change of I-HCl after heating at 100° was due to its decompn. to berberubine.

Berberine-induced bile bilirubin secretion in the rat

Berberine-induced bile bilirubin secretion in the rat. Hobara, Norio; Watanabe, Akiharu (Med. Sch., Okayama Univ., Okayama, Japan). Curr. Ther. Res., 35(4), 663-7 (English) 1984. CODEN: CTCEA9. ISSN: 0011-393X. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Increased bile bilirubin [635-65-4] output and concn. were obsd. after oral and s.c. administration of berberine [2086-83-1] to rats; i.v. injection of the drug, even at a smaller dose, produced stronger responses. However, bile flow rate remained at basal levels. The choleretic action of berberine, as measured by bilirubin output, was similar to that of the hydrocholeretic agent a-hydroxy-1-cyclohexylbutyric acid.