Reference

L-Histidine is a beneficial adjuvant for antiepileptic drugs against maximal electroshock-induced seizures in mice

L-Histidine is a beneficial adjuvant for antiepileptic drugs against maximal electroshock-induced seizures in mice. Kaminski, R. M.; Zolkowska, D.; Kozicka, M.; Kleinrok, Z.; Czuczwar, S. J. (Isotope Laboratory, Institute of Agricultural Medicine, Lublin, Pol.). Amino Acids, 26(1), 85-89 (English) 2004 Springer-Verlag Wien. CODEN: AACIE6. ISSN: 0939-4451. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Endogenous histamine has been reported to be involved in regulation of seizure susceptibility. Enhancement of histamine neurotransmission engendered by L-histidine treatment produces anticonvulsant effects in exptl. animals. The present study investigated the influence of L-histidine on the protective effects of carbamazepine and phenytoin against maximal electroshock-induced seizures in mice. L-Histidine, administered at the doses that did not influence the threshold for electroconvulsions (250-500 mg/kg), enhanced by nearly 30% the protective effects of carbamazepine against maximal electroshock-induced seizures. D-Histidine (1000 mg/kg), an inactive isomer of histidine, was without any effect in this regard. L-Histidine (500 mg/kg) also augmented the protective effects of phenytoin. Importantly, the enhancement of the anticonvulsant effects of these antiepileptic drugs produced by L-histidine co-administration was not assocd. with augmentation of their unwanted effects on memory and motor performance. A pharmacokinetic interaction was also excluded since the free plasma levels of these antiepileptics remained unchanged in the presence of L-histidine. It may be suggested that L-histidine could serve as a beneficial adjuvant for selected antiepileptic drugs.

High-resolution polyacrylamide gel electrophoresis of oligonucleotides using L-histidine buffer

High-resolution polyacrylamide gel electrophoresis of oligonucleotides using L-histidine buffer. Mandecki, Wlodek; Hayden, Mark (Corp. Mol. Biol., Abbott Lab., Abbott Park, IL 60064, USA). DNA, 7(1), 57-62 (English) 1988. CODEN: DNAADR. ISSN: 0198-0238. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) L-Histidine (50 mM, pH 7.6) as a buffer for gel electrophoresis greatly improves the resoln. of oligodeoxyribonucleotides <70 residues long on denaturing polyacrylamide gels. The histidine buffer increases spacing between DNA bands on the gel ~2-fold in comparison with a std. buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3). In addn., low cond. of the histidine buffer results in a 3-fold redn. of the electrophoresis time. Conditions for electrophoresis were optimized by varying both histidine and acrylamide concns. Other polycationic compds., such as spermidine and ethylenediamine, were also tested for improved resoln. of oligonucleotides. Several hypotheses as to the factors influencing the sepn. of DNA on gels are presented.