Reference

Simultaneous determination of dimethadione and trimethadione by infrared-spectrometry: application for mean intracellular pH measurement

Simultaneous determination of dimethadione and trimethadione by infrared-spectrometry: application for mean intracellular pH measurement. Zweens, J.; Frankena, Henny (Dep. Physiol., Univ. Groningen, Groningen NL-9712 KZ, Neth.). J. Clin. Chem. Clin. Biochem., 22(10), 641-5 (English) 1984. CODEN: JCCBDT. ISSN: 0340-076X. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) A simple IR-spectrometric method for the simultaneous detn. of dimethadione [695-53-4] and trimethadione [127-48-0] in plasma is described. The method is based on the absorption band of the carbonyl group for trimethadione at the wavenumber of 1740 cm-1 and for dimethadione at the wavenumber of 1770 cm-1. The accuracy and precision of the method are excellent; at a dimethadione and trimethadione concn. of 0.5 mmol×L-1 the coeff. of variation for the detn. of both compds. is <1%, which is better than that for the UV spectrometric or gas-liq. chromatog. methods. The method can be used for the detn. of the ratio trimethadione/dimethadione in the clin. setting as a sensitive check of the patient's adherence to therapy. It can also be used for the detn. of dimethadione as an indicator for the mean body intracellular pH.

The protective effect of cysteine on chemical-induced liver injury in rats

The protective effect of cysteine on chemical-induced liver injury in rats. Tanaka, Einosuke; Hagino, Sigenobu; Yoshida, Takemi; Kuroiwa, Yukio; Ohno, Hiromitsu; Numata, Hiroshi (Sch. Pharm. Sci., Showa Univ., Tokyo 142, Japan). J. Toxicol. Sci., 9(3), 245-51 (English) 1984. CODEN: JTSCDR. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Section cross-reference(s): 1 The possible protective effect of cysteine [52-90-4] on chem.-induced liver injury was studied in rats in vivo and in vitro. There was no increase in the activity of serum glutamic-oxaloacetic transaminase (GOT) [9000-97-9] of rats pretreated with cysteine (1.2 g/kg, orally) followed by 0.25 mL/kg CCl4 [56-23-5], D-galactosamine (GalN) [7535-00-4], or a-naphthylisothiocyanate (ANIT) [551-06-4]. However, rats pretreated with cysteine followed by 0.5 mL/kg CCl4 were not protected. The content of cytochrome P 450 [9035-51-2], activity of aminopyrine N-demethylase [9037-69-8], or serum ratio of 5,5-dimethyl-2,4-oxazolidinedione (DMO) [695-53-4] to trimethadione (TMO) [127-48-0] (DMO/TMO ratio) after CCl4, GalN, or ANIT were not altered by pretreatment with cysteine. However, pretreatment with cysteine prevented changes in the content of cytochrome P 450, activity of aminopyrine N-demethylase, and DMO/TMO ratio in serum as well as the activities of serum GOT and GPT [9000-86-6] when the rats were treated with bromobenzene (BZ) [108-86-1]. The degree of lipid peroxidn. from CCl4 was markedly reduced by the presence of 10-4M cysteine. Thus, cysteine may have a protective effect on chem.-induced liver injury produced via epoxide metabolites.