133324-02-4 Usage
Description
2'-Deoxy-2'-fluoro-5'-O-(4,4'-dimethoxytrityl)-5-methyluridine, with the CAS number 133324-02-4, is a uridine derivative that plays a significant role in the field of molecular biology and biochemistry. It is characterized by its unique chemical structure, which includes a 2'-deoxy-2'-fluoro modification and a 5'-O-(4,4'-dimethoxytrityl) group, as well as a 5-methyl group at the uridine base. 2'-Deoxy-2'-fluoro-5'-O-(4,4'-dimethoxytrityl)-5-methyluridine is known for its stability and reactivity, making it a valuable tool in various research and diagnostic applications.
Uses
Used in Synthetic Chemistry:
2'-Deoxy-2'-fluoro-5'-O-(4,4'-dimethoxytrityl)-5-methyluridine is used as a key intermediate in the synthetic preparation of 2'-fluorinated thymidine glycol oligonucleotides. These oligonucleotides are essential for the development of inhibitors targeting the endonuclease III reaction, a critical enzyme involved in DNA repair and recombination processes. The compound's unique structure allows for the efficient synthesis of these therapeutically relevant molecules, contributing to the advancement of novel treatments for various genetic disorders and diseases.
Used in Molecular Biology Research:
In the field of molecular biology, 2'-Deoxy-2'-fluoro-5'-O-(4,4'-dimethoxytrityl)-5-methyluridine is employed as a building block for the synthesis of modified oligonucleotides with enhanced stability and specificity. These modified oligonucleotides can be used as probes or primers in various molecular biology techniques, such as polymerase chain reaction (PCR), DNA sequencing, and DNA microarray analysis. The compound's fluorinated and protected structure provides improved resistance to enzymatic degradation and allows for more accurate and reliable results in these applications.
Used in Drug Development:
The unique chemical properties of 2'-Deoxy-2'-fluoro-5'-O-(4,4'-dimethoxytrityl)-5-methyluridine make it a promising candidate for the development of new drugs targeting DNA repair pathways. By inhibiting the endonuclease III reaction, this compound and its derivatives have the potential to disrupt the DNA repair mechanisms in cancer cells, leading to increased sensitivity to radiation and chemotherapy. This could result in the development of more effective cancer treatments with fewer side effects.
Used in Diagnostic Applications:
In the diagnostic industry, 2'-Deoxy-2'-fluoro-5'-O-(4,4'-dimethoxytrityl)-5-methyluridine can be utilized as a component in the development of novel diagnostic tools and assays. Its stability and reactivity make it suitable for the design of highly specific and sensitive detection systems for various genetic and infectious diseases. By incorporating this compound into diagnostic platforms, researchers and clinicians can improve the accuracy and efficiency of disease detection, leading to better patient outcomes and more effective treatment strategies.
Check Digit Verification of cas no
The CAS Registry Mumber 133324-02-4 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,3,3,3,2 and 4 respectively; the second part has 2 digits, 0 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 133324-02:
(8*1)+(7*3)+(6*3)+(5*3)+(4*2)+(3*4)+(2*0)+(1*2)=84
84 % 10 = 4
So 133324-02-4 is a valid CAS Registry Number.
133324-02-4Relevant articles and documents
A minute amount of S-puckered sugars is sufficient for (6-4) photoproduct formation at the dinucleotide level
Moriou, Ceìline,Denhez, Cleìment,Plashkevych, Oleksandr,Coantic-Castex, Steìphanie,Chattopadhyaya, Jyoti,Guillaume, Dominique,Clivio, Pascale
, p. 615 - 619 (2015)
The di-2′-α-fluoro analogue of thymidylyl(3′,5′)-thymidine, synthesized to probe the effect of a minimum amount of S conformer on the photoreactivity of dinucleotides, is endowed with only 3% and 8% of S sugar conformation at its 5′- and 3′-end, respectively. This analogue gives rise to the (6-4) photoproduct as efficiently as the dithymine dinucleotide (74% and 66% at the 5′- and 3′-end, respectively) under 254 nm. Our results suggest that the 5′-N, 3′-S conformer gives rise to the (6-4) photoproduct.
S-ANTIGEN TRANSPORT INHIBITING OLIGONUCLEOTIDE POLYMERS AND METHODS
-
, (2021/06/22)
Various embodiments provide STOPS? polymers that are S-antigen transport inhibiting oligonucleotide polymers, processes for making them and methods of using them to treat diseases and conditions. In some embodiments the STOPS? modified oligonucleotides include an at least partially phosphorothioated sequence of alternating A and C units having modifications as described herein. The sequence independent antiviral activity against hepatitis B of embodiments of STOPS? modified oligonucleotides, as determined by HBsAg Secretion Assay, is an EC50 that is less than 100 nM.
Synthesis and anti-HIV activity of 2′-deoxy-2′-fluoro-4′-C-ethynyl nucleoside analogs
Wang, Qiang,Li, Yanfeng,Song, Chuanjun,Qian, Keduo,Chen, Chin-Ho,Lee, Kuo-Hsiung,Chang, Junbiao
supporting information; experimental part, p. 4053 - 4056 (2010/08/19)
Based on the favorable antiviral profiles of 4′-substituted nucleosides, novel 1-(2′-deoxy-2′-fluoro-4′-C-ethynyl-β-d-arabinofuranosyl)-uracil (1a), -thymine (1b), and -cytosine (2) analogs were synthesized. Compounds 1b and 2 exhibited potent anti-HIV-1 activity with IC50 values of 86 and 1.34 nM, respectively, without significant cytotoxicity. Compound 2 was 35-fold more potent than AZT against wild-type virus, and also retained nanomolar antiviral activity against resistant strains, NL4-3 (K101E) and RTMDR. Thus, 2 merits further development as a novel NRTI drug.
