343-91-9

Basic information

• Product Name: 5-fluorotryptophan
• Synonyms: DL-Tryptophan,5-fluoro-; Tryptophan, 5-fluoro-, DL- (8CI); 5-Fluoro-DL-tryptophan;DL-5-Fluorotryptophan; NSC 9363
• CAS NO: 343-91-9
• Molecular Formula: C11H11 F N2 O2
• Molecular Weight: 0
• Mol File: 343-91-9.mol
• Article Data: 16

Chemical Properties

• Boiling Point: 450.7°Cat760mmHg
• Flash Point: 226.4°C
• Appearance: /
• Density: 1.442g/cm³
• CAS DataBase Reference: 5-fluorotryptophan(CAS DataBase Reference)
• NIST Chemistry Reference: 5-fluorotryptophan(343-91-9)
• EPA Substance Registry System: 5-fluorotryptophan(343-91-9)

Safety Data

• Hazardous Substances Data: 343-91-9(Hazardous Substances Data)

Upstream product

• 61272-76-2 4-fluoro-2-iodoaniline 
• 261177-58-6 5-fluoro-L-tryptophan ethyl ester 
• 154170-01-1 ethyl 2-amino-3-(5-fluoro-1H-indol-3-yl)propanoate 
• 1017683-44-1 (5-fluoro-3-indolylmethyl)trimethylammonium iodide 
• 399-52-0 5-fluoro-1H-indole 
• 343-90-8 3-dimethylaminomethyl-5-fluoroindole 
• 427-87-2 acetylamino-[3-(4-fluoro-phenylhydrazono)-propyl]-malonic acid diethyl ester 
• 428-79-5 acetylamino-(5-fluoro-indol-3-ylmethyl)-malonic acid diethyl ester 
• 363-37-1 acetylamino-(5-fluoro-indol-3-ylmethyl)-malonic acid 
• 154-08-5 DL-5-fluorotryptophan 
• 56-45-1 L-serin 
• 908846-88-8 (S)-2-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-3-(5-fluoro-1H-indol-3-yl)-propanoic acid 
• 114926-41-9 (S)-2-((tert-butoxycarbonyl)amino)-3-(5-fluoro-1H-indol-3-yl)propanoic acid 
• 7303-54-0 N^α-acetyl-5-fluoro-D,L-tryptophan 

Downstream Products

• 1234870-95-1 t-butoxycarbonyl-DL-6-fluorotryptophan 
• 67337-05-7 2-((tert-butoxycarbonyl)amino)-3-(5-fluoro-1H-indol-3-yl)propanoic acid 
• 343-91-9 D-5-fluorotryptophan 
• 114926-41-9 (S)-2-((tert-butoxycarbonyl)amino)-3-(5-fluoro-1H-indol-3-yl)propanoic acid 
• 908846-88-8 (S)-2-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-3-(5-fluoro-1H-indol-3-yl)-propanoic acid 
• 1403850-91-8 (2S)-N-((3S,6S)-3-(tert-butyl)-1⁹-fluoro-2⁴-(4-(hydroxymethyl)oxazol-2-yl)-5-oxo-1^5a,1^10bdihydro-1⁶H-4-aza-1(10b,2)-benzofuro[2,3-b]indola-2(5,2)-oxazolacycloheptaphane-6-yl)-2-hydroxy-3-methylbutanamide 

Relevant articles and documents

METHODS FOR PRODUCING D-TRYPTOPHAN AND SUBSTITUTED D-TRYPTOPHANS

Single-module nonribosomal peptide synthetases (NRPSs) and NRPS-like enzymes activate and transform carboxylic acids in both primary and secondary metabolism; and are of great interest due to their biocatalytic potentials. The single-module NRPS IvoA is essential for fungal pigment biosynthesis. As disclosed herein, we show that IvoA catalyzes ATP-dependent unidirectional stereoinversion of L-tryptophan to D-tryptophan with complete conversion. While the stereoinversion is catalyzed by the epimerization (E) domain, the terminal condensation (C) domain stereoselectively hydrolyzes D-tryptophanyl-S-phosphopantetheine thioester and thus represents a noncanonical C domain function. Using IvoA, we demonstrate a biocatalytic stereoinversion/deracemization route to access a variety of substituted D-tryptophan analogs in high enantiomeric excess.

One-Pot Biocatalytic Synthesis of Substituted d -Tryptophans from Indoles Enabled by an Engineered Aminotransferase

d-Tryptophan and its derivatives are important precursors of a wide range of indole-containing pharmaceuticals and natural products. Here, we developed a one-pot biocatalytic process enabling the synthesis of d-tryptophans from indoles in good yields and high enantiomeric excess (91% to >99%). Our method couples the synthesis of l-tryptophans catalyzed by Salmonella enterica tryptophan synthase with a stereoinversion cascade mediated by Proteus myxofaciens l-amino acid deaminase and an aminotransferase variant that we engineered to display native-like activity toward d-tryptophan. Our process is applicable to preparative-scale synthesis of a broad range of d-tryptophan derivatives containing electron-donating or -withdrawing substituents at all benzene-ring positions on the indole group.

Biosynthesis of violacein, structure and function of L-tryptophan oxidase VioA from chromobacterium violaceum

Violacein is a natural purple pigment of Chromobacterium violaceum with potential medical applications as antimicrobial, antiviral, and anticancer drugs. The initial step of violacein biosynthesis is the oxidative conversion of L-tryptophan into the corresponding α-imine catalyzed by the flavoenzyme L-tryptophan oxidase (VioA). A substrate-related (3-(1H-indol-3-yl)-2-methylpropanoic acid) and a product-related (2-(1H-indol-3-ylmethyl)prop-2-enoic acid) competitive VioA inhibitor was synthesized for subsequent kinetic and x-ray crystallographic investigations. Structures of the binary VioA?FADH2 and of the ternary VioA?FADH2 ?2-(1H-indol-3-ylmethyl)prop-2-enoic acid complex were resolved. VioA forms a "loosely associated" homodimer as indicated by small-angle x-ray scattering experiments. VioA belongs to the glutathione reductase family 2 of FAD-dependent oxidoreductases according to the structurally conserved cofactor binding domain. The substrate-binding domain of VioA is mainly responsible for the specific recognition of L-tryptophan. Other canonical amino acids were efficiently discriminated with a minor conversion of L-phenylalanine. Furthermore, 7-aza-tryptophan, 1-methyl-tryptophan, 5-methyl-tryptophan, and 5-fluoro-tryptophan were efficient substrates of VioA. The ternary product-related VioA structure indicated involvement of protein domain movement during enzyme catalysis. Extensive structure-based mutagenesis in combination with enzyme kinetics (using L-tryptophan and substrate analogs) identified Arg64 , Lys269 , and Tyr309 as key catalytic residues of VioA. An increased enzyme activity of protein variant H163A in the presence of L-phenylalanine indicated a functional role of His163 in substrate binding. The combined structural and mutational analyses lead to the detailed understanding of VioA substrate recognition. Related strategies for the in vivo synthesis of novel violacein derivatives are discussed.