56-84-8 Usage
Chemical Properties
Different sources of media describe the Chemical Properties of 56-84-8 differently. You can refer to the following data:
1. The product is white crystal or crystalline powder, slightly sour in taste. Soluble in boiling water, slightly soluble in water at 25℃(0.5%), freely soluble in dilute acid and sodium hydroxide solution and insoluble in ethanol and ethyl ether. It decomposes when heated to 270 ℃. Its isoelectric point is 2.77 and its specific rotation is associated with the soluble solvent. It is dextral in the acid solution and water solution, but levorotary in the alkali solution. [α]25D+5.05 (C=0.5-2.0 g/ml, H2O). Combined with HNO2, alcohol or acyl chloride, it can produce L- malic acid, ester and amide respectively. It is the ingredient of unripe sugarcane and beet molasses.
2. Colorless crystals. Soluble in water; insoluble in alcohol and ether. Optically active. dl-aspartic acid.
3. Aspartic acid (abbreviated as Asp or D) is an α-amino acid with the chemical formula HOOCCH(NH2 )CH2COOH. The carboxylate anion, salt, or ester of aspartic acid is known as aspartate. The Lisomer of aspartate is one of the 20 proteinogenic amino acids, i.e., the building blocks of proteins. Its codons are GAU and GAC. Aspartic acid is, together with glutamic acid, classified as an acidic amino acid with a pKa of 3.9, however in a peptide the pKa is highly dependent on the local environment. A pKa as high as 14 is not at all uncommon. Aspartate is pervasive in biosynthesis. As with all amino acids, the presence of acid protons depends on the residue's local chemical environment and the pH of the solution.
4. Apartic acid is an aliphatic monoaminodicarboxylic acid (amino acid) and is a well-known constituent of protein. It
has a slight acid taste
Uses
Different sources of media describe the Uses of 56-84-8 differently. You can refer to the following data:
1. It is used as an electrolyte supplement for aminophenol transfusion, inorganic ion supplement (K+, Ca+, etc.) and fatigue restorer. Potassium magnesium aspartate injection or oral solution can be used for arrhythmia, premature beat, tachycardia, hypokalemia, hypomagnesemia, heart failure, myocardial infarction, angina pectoris, hepatitis, cirrhosis and other diseases caused by cardiac glycoside poisoning. Due to its low toxicity, this product cannot be injected without dilution, and the patients with renal insufficiency and atrioventricular conduction block should use it with caution.
2. L-Aspartic acid is used as a component of parenteral and enteral nutrition and as a pharmaceutical ingredient. it is used for cell culture and in manufacturing processes. It is widely utilized for mineral supplementation in the salt form.
3. L-aspartic acid is used as a dietary supplement, it can be blended with minerals to make compounds like potassium aspartate, copper aspartate, manganese aspartate, magnesium aspartate, zinc aspartate and more. Increasing the absorption, and hence utilization potentials, of these minerals via the addition of aspartate induces certain health benefits. Many athletes use L-aspartic acid-based mineral supplements orally to enhance their performance capacities.
Aspartic acid and glutamic acid play important roles as general acids in enzyme active centers, as well as in maintaining the solubility and ionic character of proteins. It can help promote a robust metabolism, and is sometimes used to treat fatigue and depression.
Aspartic acid is used as a component of parenteral and enteral nutrition. In pharmaceutical agents aspartic acid is used as an ammoniac detoxicating agent, hepar function accelerator and fatigue refresher.
4. l-aspartic acid is an amino acid used as a skin-conditioning agent.
Production
L- aspartic acid is mainly produced by enzymatic method. L- aspartase acts on the fumaric acid and ammonia, that is, which generates L- aspartic acid.
Strain Culturing: Eschrichia coli Asl.881 was cultured. The common meat juice medium is agarslantculture-medium. The vase medium comprises corn steep liquor 7.5%, fumaric acid 2.0% and MgSO4 7H2O 0.02%. Adjust the pH value of solution to 6.0 with ammonia water, then put 50-100ml culture medium into 500ml cone bottle after boiling and filtering. Take the fresh cultivated seeds on the slope or in the liquid, inoculate culture medium in shake flask, shake overnight at 37℃, adjust pH to 5.0 with 1mol/L HCl after enlarging culture step by step to 1000-2000L, cool it to room temperature after keeping 45℃ for 1h, centrifuge in rotary supercentrifuge and collect the thallus including aspartase.
Immobilize aspartase: Make a bioreactor to take out 20kg E.Coli wet cell, suspend it in the culture supernatant after centrifugation in 80L (or 80L saline), keep it at 40℃ and then add 90L 12% gelatin solution and 1.0% glutaraldehyde solution, which should be held 40℃. ?Stir well, set aside to cool down and solidify, soak in 0.25% glutaraldehyde solution, hold 5℃ after an overnight, cut into small pieces ( 3-5 mm3) , soak in 0.25% glutaraldehyde solution at 5℃ for a night, take it out and elut with water, drain to obtain immobilized E.Coli containing aspartase and load it into filled bioreactor in reserve.
Conversion: The solution of 1 mol/L of ammonium fumarate (including 1mmol/L MgCl2, pH8.5) substrate, which keeps 37℃, flows through a bioreactor at a constant speed (SV) continuously in the case of controlling the maximum conversion rate over 95% and then the conversion solution is obtained.
Roughhew and refine: ?Add 1 mol/L of HCl into the conversion solution gradually to adjust pH valkue to 2.8, place at 5℃ overnight for crystallizing, filter to prepare crystallization, drain after water washing, drying at 105℃ to obtain L- aspartic acid crude. Use dilute ammonia to recrystallize, dissolve into 15% solution (pH5.0) with ammonia, add 1% activated carbon, stir and fade for 1h when heat to 70℃, filter immediately to remove slag, cool the filtrate, hold 5℃ overnight for crystallizing, filter to get crystallization and obtain the L-Aspartic acid finished products after vacuum drying at 85℃.
Description
L-Aspartic acid is the L-form of the aspartic acid. It is one of the 20 amino acids that used in the protein synthesis. It is the non-essential amino acids for humans as it can be synthesized in vivo. It is important in the synthesis of other amino acids and some nucleotides, and is a metabolite in the citric acid and urea cycles. In animals, it may be used as a neurotransmitter. It can be chemically synthesize from the diethyl sodium phthalimidomalonate. Currently, almost all the aspartic acids are manufactured in China. Its application include being used as low calorie sweetener (as the part of the aspartame), scale and corrosion inhibitor, and in resins. One of its growing applications is for the manufacturing of biodegradable superabsorbent polymer, polyaspartic acid. It can also be used in fertilizer industry to improve water retention and nitrogen uptake.
Occurrence
Dietary sources Aspartic acid is not an essential amino acid, which means that it can be synthesized from central metabolic pathway intermediates in humans. Aspartic acid is found in : Animal sources : luncheon meats, sausage meat, wild game Vegetable sources: sprouting seeds, oat flakes, avocado, asparagus , young sugarcane, and molasses from sugar beets. Chemical synthesis Racemic aspartic acid can be synthesized from diethyl sodium phthalimido malonate, (C6H4(CO)2NC(CO2Et)2). The major disadvantage of the above technique is that equimolar amounts of each enantiomer are made. Using biotechnology it is now possible to use immobilized enzymes to create just one type of enantiomer owing to their stereo specificity. Aspartic acid is made synthetically using ammonium fumarate and aspartase from E.coli, E.coli usually breaks down the aspartic acid as a nitrogen source but using excess amounts of ammonium fumarate a reversal of the enzyme's job is possible, and so aspartic acid is made to very high yields, 98.7 mM from 1 M.
History
Aspartic acid was first discovered in 1827 by Plisson, derived from asparagine, which had been isolated from asparagus juice in 1806, by boiling with a base.
Definition
ChEBI: The L-enantiomer of aspartic acid.
General Description
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Hazard
Low toxicity.
Biological Activity
Endogenous NMDA receptor agonist.
Safety Profile
Low toxicity by
intraperitoneal route. When heated to
decomposition emits toxic fumes of NOx.
Synthesis
Different sources of media describe the Synthesis of 56-84-8 differently. You can refer to the following data:
1. Enzymatically, aspartic acid is reversibly synthesized by a transamination reaction between oxaloacetic acid and glutamic
acid in the presence of pyridoxal phosphate.
2. Aspartate is non - essential in mammals, being produced from oxaloacetate by transamination. It can also be generated from ornithine and citrulline in the urea cycle. In plants and microorganisms, aspartate is the precursor to several amino acids, including four that are essential for humans: methionine, threonine, isoleucine, and lysine. The conversion of aspartate to these other amino acids begins with reduction of aspartate to its "semi aldehyde," O2CCH(NH2)CH2CHO. Asparagine is derived from aspartate via trans amidation : -O2CCH(NH2)CH2CO2 - + G C (O)NH3+ O2CCH(NH2)CH2CONH3+ + GC(O)O (where GC(O)NH2 and GC(O)OH are glutamine and glutamic acid, respectively).
Forms and nomenclature
There are two forms or enantiomers of aspartic acid. The name "aspartic acid" can refer to either enantiomer or a mixture of two. Of these two forms, only one, "L - aspartic acid", is directly incorporated into proteins. The biological roles of its counterpart, "Daspartic acid" are more limited. Where enzymatic synthesis will produce one or the other, most chemical syntheses will produce both forms, "DL-aspartic acid," known as a racemic mixture.
Other biochemical roles
Aspartate is also a metabolite in the urea cycle and participates in gluconeogenesis. It carries reducing equivalents in the malateaspartate shuttle, which utilizes the ready inter conversion of aspartate and oxaloacetate, which is the oxidized (dehydrogenated) derivative of malic acid. Aspartate donates one nitrogen atom in the biosynthesis of inosine, the precursor to the purine bases. In addition, aspartic acid acts as hydrogen acceptor in a chain of ATP synthase.
Check Digit Verification of cas no
The CAS Registry Mumber 56-84-8 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 5 and 6 respectively; the second part has 2 digits, 8 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 56-84:
(4*5)+(3*6)+(2*8)+(1*4)=58
58 % 10 = 8
So 56-84-8 is a valid CAS Registry Number.
InChI:InChI=1/C4H7NO4/c5-2(4(8)9)1-3(6)7/h2H,1,5H2,(H,6,7)(H,8,9)/t2-/m0/s1
56-84-8Relevant articles and documents
Structures and antitumor activities of ten new and twenty known surfactins from the deep-sea bacterium Limimaricola sp. SCSIO 53532
Chen, Min,Chen, Rouwen,Ding, Wenping,Li, Yanqun,Tian, Xinpeng,Yin, Hao,Zhang, Si
, (2022/01/11)
Surfactins are natural biosurfactants with myriad potential applications in the areas of healthcare and environment. However, surfactins were almost exclusively produced by the bacterium Bacillus species in previous reported literatures, together with difficulty in isolating pure monomer, which resulted in making extensive effort to remove duplication and little discovery of new surfactins in recent years. In the present study, the result of Molecular Networking indicated that Limimaricola sp. SCSIO 53532 might well be a potential resource for surfacin-like compounds based on OSMAC strategy. To search for new surfactins with significant biological activity, further study was undertaken on the strain. As a result, ten new surfactins (1–10), along with twenty known surfactins (11–30), were isolated from the ethyl acetate extract of SCSIO 53532. Their chemical structures were established by detailed 1D and 2D NMR spectroscopy, HRESIMS data, secondary ion mass spectrometry (MS/MS) analysis, and chemical degradation (Marfey's method) analysis. Cytotoxic activities of twenty-seven compounds against five human tumor cell lines were tested, and five compounds showed significant antitumor activities with IC50 values less than 10 μM. Furtherly, analysis of structure–activity relationships revealed that the branch of side chain, the esterification of Glu or Asp residue, and the amino acid residue of position 7 possessed a great influence on antitumor activity.
Biosynthesis ofl-alanine fromcis-butenedioic anhydride catalyzed by a triple-enzyme cascadeviaa genetically modified strain
Cui, Ruizhi,Liu, Zhongmei,Yu, Puyi,Zhou, Li,Zhou, Zhemin
, p. 7290 - 7298 (2021/09/28)
In industry,l-alanine is biosynthesized using fermentation methods or catalyzed froml-aspartic acid by aspartate β-decarboxylase (ASD). In this study, a triple-enzyme system was developed to biosynthesizel-alanine fromcis-butenedioic anhydride, which was cost-efficient and could overcome the shortcomings of fermentation. Maleic acid formed bycis-butenedioic anhydride dissolving in water was transformed tol-alanineviafumaric acid andl-asparagic acid catalyzed by maleate isomerase (MaiA), aspartase (AspA) and ASD, respectively. The enzymatic properties of ASD from different origins were investigated and compared, as ASD was the key enzyme of the triple-enzyme cascade. Based on cofactor dependence and cooperation with the other two enzymes, a suitable ASD was chosen. Two of the three enzymes, MaiA and ASD, were recombinant enzymes cloned into a dual-promoter plasmid for overexpression; another enzyme, AspA, was the genomic enzyme of the host cell, in which AspA was enhanced by a T7promoter. Two fumarases in the host cell genome were deleted to improve the utilization of the intermediate fumaric acid. The conversion of whole-cell catalysis achieved 94.9% in 6 h, and the productivity given in our system was 28.2 g (L h)?1, which was higher than the productivity that had been reported. A catalysis-extraction circulation process for the synthesis ofl-alanine was established based on high-density fermentation, and the wastewater generated by this process was less than 34% of that by the fermentation process. Our results not only established a new green manufacturing process forl-alanine production fromcis-butenedioic anhydride but also provided a promising strategy that could consider both catalytic ability and cell growth burden for multi-enzyme cascade catalysis.
Noncovalently Functionalized Commodity Polymers as Tailor-Made Additives for Stereoselective Crystallization
Wan, Xinhua,Wang, Zhaoxu,Ye, Xichong,Zhang, Jie
supporting information, p. 20243 - 20248 (2021/08/09)
Stereoselective inhibition of the nucleation and crystal growth of one enantiomer aided by “tailor-made” polymeric additives is an efficient method to obtain enantiopure compounds. However, the conventional preparation of polymeric additives from chiral monomers are laborious and limited in structures, which impedes their rapid optimization and applicability. Herein, we report a “plug-and-play” strategy to facilitate synthesis by using commercially available achiral polymers as the platform to attach various chiral small molecules as the recognition side-chains through non-covalent interactions. A library of supramolecular polymers made up of two vinyl polymers and six small molecules were applied with seeds in the selective crystallization of seven racemates in different solvents. They showed good to excellent stereoselectivity in yielding crystals with high enantiomeric purities in conglomerates and racemic compound forming systems. This convenient, low-cost modular synthesis strategy of polymeric additives will allow for high-efficient, economical resolution of various racemates on different scales.